Analysis unit, biosensor and method for detecting or determining the concentration of an analyte

• US 20080153710A1
• Filed: 05/03/2007
• Published: 06/26/2008
• Est. Priority Date: 05/04/2006
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Hengerer, Arne, Kuth, Rainer
• Application Number:
  US11/797,428
• Publication Number:
  US 20080153710A1
• Time in Patent Office: Days
• US Class Current: 506/6
• CPC Class Codes: G01N 2291/0222 Binary liquids
• CPC Class Codes: G01N 2291/0255 (Bio)chemical reactions, e.g. on biosensors
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/106 one or more transducer arrays
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves

Abstract

A biosensor includes an analysis unit and a reader device for detecting an analyte. In at least one embodiment, the analysis unit includes a quartz oscillator on whose surface capture molecules, which bind specifically to the analyte, are immobilized; as well as a transponder to transmit information, regarding whether and/or how many analyte molecules have bound to the capture molecules, to the reader device.

Claims

1. An analysis unit for at least one of detecting and determining the concentration of an analyte, the analysis unit comprising:

at least one sensor with a quartz oscillator, on whose surface capture molecules which bind specifically to the analyte are immobilized, a natural frequency of the quartz oscillator depending on at least one of whether and how many analyte molecules are bound to the capture molecules; and

a transponder for wireless information interchange with a reader device, electrically connected to the quartz oscillator and designed to transmit information to the reader device, regarding at least one of whether and how many analyte molecules have bound to the capture molecules, the at least one sensor including three quartz oscillators, the first quartz oscillator being coated with first control molecules whose molecular weight corresponds to that of the capture molecules, the second quartz oscillator being coated with second control molecules whose molecular weight corresponds to a complex of a capture molecule and an analyte molecule, and the third quartz oscillator being coated with capture molecules.

2. The analysis unit as claimed in claim 1, wherein the transponder is passive.

3. The analysis unit as claimed in claim 1, wherein the transponder is active and includes a power supply.

4. The analysis unit as claimed in claim 1, wherein the transponder comprises a microchip and an antenna.

5. The analysis unit as claimed in claim 1, wherein the analysis unit comprises a separate transponder for each of the three quartz oscillators.

6. The analysis unit as claimed in claim 1, wherein the analysis unit comprises a plurality of sensors, on whose quartz oscillators capture molecules which are specific to different analytes are respectively immobilized.

7. The analysis unit as claimed in claim 1, wherein the capture molecules are biopolymers, in particular nucleic acids, proteins, peptides, polysaccharides, antibodies or antibody fragments, or viruses or bacteria.

8. A biosensor for at least one of detecting and determining the concentration of an analyte, comprising:

an analysis unit as claimed in claim 1; and

a reader device, to interchange information with the transponder of the analysis unit via electromagnetic waves.

9. The biosensor as claimed in claim 8, wherein the reader device includes an antenna, by which electromagnetic waves are generateable whose frequency corresponds at least approximately to the natural frequency of the quartz oscillators.

10. The biosensor as claimed in claim 9, wherein the electromagnetic waves have a frequency spectrum which contains the natural frequencies of the quartz oscillators both with and without analyte molecules bound to the capture molecules.

11. The biosensor as claimed in claim 8, wherein the reader device comprises an antenna by which at least one of an electromagnetic RF and UHF field is generateable, receivable by the antenna of the transponder and by whose energy the microchip of the transponder is activateable.

13. The analysis unit as claimed in claim 2, wherein the transponder comprises a microchip and an antenna.

14. The analysis unit as claimed in claim 3, wherein the transponder comprises a microchip and an antenna.

15. The analysis unit as claimed in claim 7, wherein the biopolymers include at least one of nucleic acids, proteins, peptides, polysaccharides, antibodies, antibody fragments, viruses and bacteria.

16. A biosensor for at least one of detecting and determining the concentration of an analyte, comprising:

an analysis unit as claimed in claim 2; and

a reader device, to interchange information with the transponder of the analysis unit via electromagnetic waves.

17. A biosensor for at least one of detecting and determining the concentration of an analyte, comprising:

an analysis unit as claimed in claim 3; and

a reader device, to interchange information with the transponder of the analysis unit via electromagnetic waves.

18. The biosensor as claimed in claim 9, wherein the reader device comprises an antenna by which at least one of an electromagnetic RF and UHF field is generateable, receivable by the antenna of the transponder and by whose energy the microchip of the transponder is activateable.

19. The biosensor as claimed in claim 10, wherein the reader device comprises an antenna by which at least one of an electromagnetic RF and UHF field is generateable, receivable by the antenna of the transponder and by whose energy the microchip of the transponder is activateable.

22. A method for at least one of detecting and determining the concentration of an analyte using the biosensor of claim 8, the method comprising:

bringing quartz oscillators, of the analysis unit of the biosensor, in contact with a sample to be examined;

activating the transponder of the analysis unit via an electromagnetic field generated by the reader device, and exciting the quartz oscillators to oscillate;

determining at least one of impedances and natural frequencies of the quartz oscillators; and

transmitting information regarding at least one of the impedances and the natural frequencies of the quartz oscillators to the reader device.

23. A computer readable medium including program segments for, when executed on a computer device, causing the computer device to implement the method of claim 22.

12. A method for at least one of detecting and determining the concentration of an analyte using a biosensor, the method comprising:

bringing quartz oscillators, of an analysis unit of the biosensor, in contact with a sample to be examined;

activating a transponder of the analysis unit via an electromagnetic field generated by a reader device, and exciting the quartz oscillators to oscillate;

determining at least one of impedances and natural frequencies of the quartz oscillators; and

transmitting information regarding at least one of the impedances and the natural frequencies of the quartz oscillators to the reader device.

20. The method of claim 12, wherein the transmited information is information regarding at least one of whether and how many analyte molecules have bound to the capture molecules on the quartz oscillators.

21. A computer readable medium including program segments for, when executed on a computer device, causing the computer device to implement the method of claim 12.