METHODS FOR EMBRYONIC STEM CELL CULTURE
First Claim
Patent Images
1. A method of cell culture comprising:
- (a) providing a human embryonic stem (ES) cell encapsulated within a support matrix to form a support matrix structure, and,(b) maintaining culture by maintaining the encapsulated cell in 3-D culture in maintenance medium.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention relates to a method of cell culture comprising providing a pluripotent ES cell encapsulated within a support matrix to form a support matrix structure, maintaining the encapsulated cell in 3-D culture in maintenance medium, and optionally differentiating the encapsulated cell in 3-D culture in differentiation medium. The invention further relates to screening methods incorporating the use of encapsulated cells.
-
Citations
63 Claims
-
1. A method of cell culture comprising:
-
(a) providing a human embryonic stem (ES) cell encapsulated within a support matrix to form a support matrix structure, and, (b) maintaining culture by maintaining the encapsulated cell in 3-D culture in maintenance medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 17, 19, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 45, 46, 48, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63)
-
-
11. A method of cell culture comprising:
-
(a) providing a single ES cell or a plurality of ES cells encapsulated within a support matrix to form a support matrix structure, (b) maintaining culture by maintaining the encapsulated cell(s) in 3-D culture in maintenance medium, in conditions suitable for ES cell maintenance, (c) performing osteogenic differentiation by differentiating the encapsulated cells in 3-D culture in differentiation medium, in conditions suitable for osteogenic differentiation. - View Dependent Claims (12, 13, 14, 15, 16, 18, 44, 47, 49, 51)
-
-
20. A method of identifying a cell environment factor that is capable of modulating cell maintenance and/or cell differentiation, wherein the method comprises
(a) providing a human embryonic stem (ES) cell encapsulated within a support matrix to form a support matrix structure, (b) maintaining culture by maintaining the encapsulated cell in 3-D culture in maintenance medium. (c) incubating the encapsulated cell in the presence of the cell environment factor, and (d) assessing the effect of the cell environment factor on cell maintenance and/or differentiation.
Specification