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Methods and Compositions for Detecting Target Sequences

  • US 20080160524A1
  • Filed: 08/10/2007
  • Published: 07/03/2008
  • Est. Priority Date: 01/24/1996
  • Status: Abandoned Application
First Claim
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1. A method for detecting a target nucleic acid, comprising:

  • a) providing a sample comprising;

    i) synthetic DNA amplified from target nucleic acid by loop-mediated isothermal amplification;

    ii) oligonucleotides capable of forming an invasive cleavage structure with said synthetic DNA; and

    iii) an agent for cleaving an invasive cleavage structure;

    b) exposing said sample to conditions wherein said oligonucleotides form an invasive cleavage structure with said synthetic DNA, wherein said invasive cleavage structure is cleaved by said agent; and

    c) detecting cleavage of an invasive cleavage structure by said agent, thereby detecting the presence of said target nucleic acid.

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