Thermo-responsive blends and uses thereof

• US 20080160559A1
• Filed: 06/08/2007
• Published: 07/03/2008
• Est. Priority Date: 06/12/2006
• Status: Abandoned Application

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Dufour, Bruno, Carre, Alain R.E., Waku-Nsimba, Jean, Henry, David
• Application Number:
  US11/811,338
• Publication Number:
  US 20080160559A1
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: C08G 2650/58 Ethylene oxide or propylene oxide copolymers, e.g. pluronics
• CPC Class Codes: C08L 2666/02 Organic macromolecular compounds, natural resins, waxes or and bituminous materials
• CPC Class Codes: C08L 2666/04 Macromolecular compounds according to groups C08L7/00 - C08L49/00, or C08L55/00 - C08L57/00; Derivatives thereof
• CPC Class Codes: C08L 2666/06 Homopolymers or copolymers of unsaturated hydrocarbons; Derivatives thereof
• CPC Class Codes: C08L 33/26 Homopolymers or copolymers of acrylamide or methacrylamide
• CPC Class Codes: C08L 71/02 Polyalkylene oxides
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity
• CPC Class Codes: G01N 33/5017 for testing neoplastic activity
• CPC Class Codes: G01N 33/502 for testing non-proliferative effects
• CPC Class Codes: G01N 33/5088 of vertebrates
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: Y10T 428/31511 Of epoxy ether
• CPC Class Codes: Y10T 428/31855 Of addition polymer from unsaturated monomers

Abstract

Described herein are thermo-responsive blends. Also described herein are articles, substrates, and kits composed of thermo-responsive blends for immobilizing cells or tissue and methods of use thereof.

Claims

1. A thermo-responsive blend comprising at least one thermo-responsive polymer and at least one non-thermo-responsive/non-water soluble polymer.

2. The blend of claim 1, wherein the thermo-responsive polymer has a lower critical solution temperature or an upper critical solution temperature between 0°

C. and 85°

C.

3. The blend of claim 1, wherein the thermo-responsive polymer comprises a homopolymer or copolymer of a (meth)acrylamide, an N-substituted acrylamide, an N,N-di-substituted (meth)acrylamide, an N-substituted (meth)acrylamide, an N,N-di-substituted (meth)acrylamide, a vinyl ether, an alkyl vinyl ether, an N-vinyl caprolactam, or a combination thereof.

4. The blend of claim 1, wherein the thermo-responsive polymer comprises a homopolymer or copolymer of acrylamide, methacrylamide, N-ethyl acrylamide, N-n-propyl acrylamide, N-n-propyl methacrylamide, N-isopropyl acrylamide, N-isopropyl methacrylamide, N-cyclopropyl acrylamide, N-cyclopropyl methacrylamide, N-ethoxyethyl acrylamide, N-ethoxyethyl methacrylamide, N-tetrahydrofurfuryl acrylamide, N-tetrahydro furfuryl methacrylamide, N,N-dimethyl (meth)acrylamide, N,N-ethylmethyl acrylamide, N,N-diethyl acrylamide, 1-(1-oxo-2-propenyl)-pyrrolidine, 1-(1-oxo-2-propenyl)-piperidine, 4-(1-oxo-2-propenyl)-morpholine, 1-(1-oxo-2-methyl-2-propenyl)-pyrrolidine, 1-(1-oxo-2-methyl-2-propenyl)-piperidine, 4-(1-oxo-2-methyl-2-propenyl)-morpholine, methyl vinyl ether, or a combination thereof.

5. The blend of claim 1, wherein the thermo-responsive polymer comprises a homopolymer of N-isopropyl acrylamide.

6. The blend of claim 1, wherein the thermo-responsive polymer comprises polyethylene oxide, polypropylene oxide, a block copolymer of polyethylene oxide-polypropylene oxide, or a cellulose ether derivative.

7. The blend of claim 1, wherein the non-thermo-responsive/non-water soluble polymer comprises a polyester, a polyamide, a polycarbonate, a polycaprolactone, a polyurethane, a polyimide, a polyamide-imide, a poly-lactide-co-glycolide, a polyolefin, a cyclic polyolefin, a polysulfone, a poly (meth)acrylate polymer, a poly vinyl polymer, or a combination thereof.

8. The blend of claim 1, wherein the thermo-responsive polymer comprises a homopolymer of N-isopropyl acrylamide and the non-thermo-responsive/non-water soluble polymer comprises polystyrene.

9. The blend of claim 1, wherein the thermo-responsive polymer comprises at least one hydrophobic unit.

10. The blend of claim 9, wherein the hydrophobic unit is derived from H₂C═

CHR, wherein R comprises an alkyl group, an aryl group, an alkoxy group, an amide group, or an ester group.

11. The blend of claim 9, wherein the hydrophobic unit is derived from styrene.

12. The blend of claim 1, wherein the thermo-responsive polymer comprises a copolymer of (1) N-isopropyl acrylamide, polyethylene oxide, a polypropylene oxide, or a block copolymer of polyethylene oxide-polypropylene oxide, or a combination thereof and (2) styrene.

13. The blend of claim 1, wherein the thermo-responsive polymer comprises a block copolymer of poly(propylene oxide)-poly(ethylene oxide)-polystyrene, a block copolymer of poly(propylene oxide)-polystyrene, or a block copolymer of poly(N-isopropylacrylamide)-polystyrene.

14. A substrate for immobilizing cells or tissue, wherein the substrate comprises the blend of claim 1.

15. An article comprising the blend of claim 1.

16. A substrate for immobilizing cells or tissue, wherein the substrate comprises the blend of claim 9.

17. An article comprising the blend of claim 9.

18. A method for growing a plurality of cells, comprising (a) depositing a parent set of cells on the substrate of claim 14, and (b) culturing the substrate with the deposited cells to promote the growth of the cells.

19. A method for growing a plurality of cells, comprising (a) depositing a parent set of cells on the substrate of claim 16, and (b) culturing the substrate with the deposited cells to promote the growth of the cells.

20. A method for differentiating cells, comprising (a) depositing a parent set of cells on the substrate of claim 14, and (b) culturing the substrate to promote differentiation of the cells.

21. A method for determining an interaction between a known cell line and a drug, comprising (a) depositing the known cell line on the substrate of claim 14;

(b) contacting the deposited cells with the drug; and

(c) identifying a response produced by the deposited cells upon contact with the drug.

22. A method for separating a compound present in a solution, comprising (a) contacting the substrate of claim 14 with the solution, wherein the compound is immobilized on the substrate;

and (b) removing the immobilized compound from the substrate.

23. A method for growing tissue, comprising (a) depositing a parent set of cells that are a precursor to the tissue on the substrate of claim 14, and (b) culturing the substrate with the deposited cells to promote the growth of the tissue.

24. A nanofiber comprising the blend of claim 1.

25. A nanofiber comprising the blend of claim 9.

26. A substrate for immobilizing cells or tissue comprising:

a. a network of nanofibers, wherein the network of nanofibers comprises one or more blends of claim 1; and

b. a base substrate comprising a first outer surface, wherein the network of nanofibers is adjacent to the first outer surface of the base substrate.

27. A substrate for immobilizing cells or tissue comprising:

a. a network of nanofibers; and

b. a base substrate comprising a first outer surface, wherein the network of nanofibers is adjacent to the first outer surface of the base substrate, and wherein the base substrate comprises one or more blends of claim 1.

28. A kit comprising (1) a network of nanofibers and (2) a base substrate, wherein the network of nanofibers, the base, or both the network of nanofibers and the base comprises one or more blends of claim 1.

29. A thermo-responsive polymer comprising at least one hydrophobic unit.