Method and device for biomolecule preparation and detection using magnetic array
First Claim
1. A method of moving magnetic particles comprisingproviding magnetic particles in a fluidic network comprising a plurality of fluidic zones, andactivating a microcoil array functionally coupled to the network to thereby move the magnetic particles without active fluidic movement in the plurality of the fluidic zones.
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Abstract
An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
72 Citations
28 Claims
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1. A method of moving magnetic particles comprising
providing magnetic particles in a fluidic network comprising a plurality of fluidic zones, and activating a microcoil array functionally coupled to the network to thereby move the magnetic particles without active fluidic movement in the plurality of the fluidic zones.
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14. A method of detecting an analyte, comprising
providing a fluidic network comprising a plurality of fluidic zones, wherein one fluidic zone comprises a magnetic affinity complex, introducing a sample suspected of comprising an analyte into a fluidic zone, wherein the magnetic affinity complex binds to the analyte to form a magnetic binding complex, activating a microcoil array functionally coupled to the network to thereby move the magnetic binding complex to a different fluidic zone without active fluidic movement in the plurality of the fluidic zones, and detecting the magnetic binding complex using a detection element functionally coupled to the network, wherein the presence of the complex indicates the presence of the analyte.
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15. A method of detecting an analyte, comprising,
providing a fluidic network comprising a plurality of fluidic zones, wherein a magnetic affinity complex and a signal affinity complex are present in one or more fluidic zones, and wherein the network is functionally coupled to an array of microcoils, introducing a sample suspected of comprising an analyte into a fluidic zone, combining the analyte with the magnetic affinity complex and the signal affinity complex, wherein the combination occurs simultaneously or sequentially, and wherein the magnetic affinity complex and the signal affinity complex bind to the analyte to form a sandwich binding complex, activating an array of microcoils functionally coupled to the fluidic network to move the sandwich binding complex within the network without active fluidic movement in the plurality of the fluidic zones, and detecting the sandwich binding complex using a detection element functionally coupled to the fluidic network, wherein the detection of the sandwich binding complex indicates the presence of the analyte.
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17. A method of detecting an analyte, comprising,
providing a fluidic network comprising a plurality of fluidic zones, wherein a magnetic affinity complex and a signal analyte complex are present in one or more fluidic zones, introducing a sample suspected of comprising an analyte into a fluidic zone, combining the analyte with the magnetic affinity complex and the signal affinity complex, wherein the magnetic affinity complex binds to the analyte to form a magnetic binding complex, activating an array of microcoils functionally coupled to the fluidic network to move the magnetic binding complex within the network without active fluidic movement in the plurality of the fluidic zones, displacing the analyte from the magnetic binding complex with the signal analyte complex to form a competitive binding complex, detecting the competitive binding complex and/or the unbound signal analyte complex using a detection element functionally coupled to the fluidic network, wherein the detection of the unbound signal analyte complex or competitive binding complex indicates the presence of the analyte.
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19. A method of detecting an analyte, comprising,
providing a fluidic network comprising a plurality of fluidic zones, wherein a coded magnetic affinity complex and a magnetic signal affinity complex are present in one or more fluidic zones, and wherein the network is functionally coupled to an array of microcoils, introducing a sample suspected of comprising an analyte into a fluidic zone, combining the analyte with the coded magnetic affinity complex, wherein the analyte binds to the coded magnetic affinity complex to form a coded magnetic binding complex, activating an array of microcoils functionally coupled to the fluidic network to move the coded magnetic binding complex within the network to a first affinity surface without active fluidic movement in the plurality of the fluidic zones, forming a bound coded magnetic binding complex, detaching the code from the bound coded magnetic binding complex, providing a magnetic signal affinity complex, wherein the detached code binds to the magnetic signal affinity complex to form a coded magnetic signal binding complex, and activating the microcoil array to move the coded magnetic signal binding complex to the detection zone comprising a second affinity surface, forming a bound coded magnetic signal binding complex, and detecting the bound coded magnetic signal binding complex using a detection element functionally coupled to the fluidic network, wherein the detection of the bound coded magnetic signal binding complex indicates the presence of the analyte.
- 20. A composition comprising an analyte bound to a magnetic affinity complex and a signal affinity complex.
Specification