Functionalized Microcantilever Sensor and Associated Method For Detection of Targeted Analytes
First Claim
1. A microcantilever sensor for targeted analyte detection comprising:
- a. a microcantilever having a base and a beam;
b. a metallic coating disposed substantially only on a first surface of a distal-most end of said beam;
c. a receptor compound immobilized to said metallic coating; and
d. said receptor compound having substantially exclusive binding interaction with said analyte.
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Accused Products
Abstract
A microcantilever sensor for targeted analyte detection can generally comprise a microcantilever having a base and a beam, a metallic coating disposed substantially only on a first surface of a distal-most end of the beam, and a receptor compound immobilized to the metallic coating wherein the receptor compound can have substantially exclusive binding interaction with the analyte. The receptor compound can be a thiol-terminated bifunctional compound having a receptor site with specific binding affinity for the analyte, for example, an isolated Fab′ fragment. The metallic coating can be a noble metal and/or a semi-noble metal, such as a bilayer of chromium and gold. The metallic coating can be applied to the microcantilever surface by electron-beam lithography.
24 Citations
34 Claims
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1. A microcantilever sensor for targeted analyte detection comprising:
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a. a microcantilever having a base and a beam; b. a metallic coating disposed substantially only on a first surface of a distal-most end of said beam; c. a receptor compound immobilized to said metallic coating; and d. said receptor compound having substantially exclusive binding interaction with said analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for functionalizing a microcantilever sensor for targeted analyte detection, the method comprising:
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a. disposing a metallic coating substantially only on a first surface of a distal-most end of said microcantilever; and b. immobilizing a receptor compound to said metallic coating, said receptor compound having substantially exclusive binding interaction with said analyte. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method for using a microcantilever sensor for detecting a targeted analyte, the method comprising:
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a. treating a first surface of a distal-most end of said microcantilever with a metallic compound; b. immobilizing a receptor compound to said metallic compound, said receptor compound having substantially exclusive binding interaction with said targeted analyte; c. exposing said first surface to a sample solution containing said targeted analyte; d. drying said first surface; and e. resonating said microcantilever in air or vacuum to detect said targeted analyte. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification