Biomolecule Immobilization on Biosensors
First Claim
1. A method for preparing a substrate for use in a biosensor, comprising:
- (a) providing a substrate surface which is reactive with an amine;
(b) coating the substrate surface with a polyamine to form a polyamine covalently linked to the substrate;
then(c) contacting the polyamine covalently linked to the substrate with a polyacid anhydride having multiple acid anhydride groups, whereby the polyacid anhydride binds to the polyamine covalently linked to the substrate with one portion of the multiple acid anhydride groups and retains another portion of the multiple acid anhydride groups for linking to amine groups on biomolecules.
2 Assignments
0 Petitions
Accused Products
Abstract
A highly specific and versatile surface chemistry for immobilization of amine-terminated probes is disclosed. A bi-layered polymer thin film serves as the platform for coupling the probes, which are preferably oligonucleotides. The process involves sequentially coating a substrate with polyamine and polyacid anhydride. Hydrolyzed polyacid anhydride groups may be converted to non-hydrolyzed groups at about 100° C. prior to probe attachment. The process of coating the substrate requires no harsh chemical pretreatment of substrates such as RCA or Piranha cleaning. In addition, simple thermal activation of the anhydride groups has a low requirement for storage, leading to a long shelf life of modified surfaces. The disclosed surface chemistry is especially compatible with microfabrication processes, and its effective application to magnetic biosensors is demonstrated.
-
Citations
25 Claims
-
1. A method for preparing a substrate for use in a biosensor, comprising:
-
(a) providing a substrate surface which is reactive with an amine; (b) coating the substrate surface with a polyamine to form a polyamine covalently linked to the substrate;
then(c) contacting the polyamine covalently linked to the substrate with a polyacid anhydride having multiple acid anhydride groups, whereby the polyacid anhydride binds to the polyamine covalently linked to the substrate with one portion of the multiple acid anhydride groups and retains another portion of the multiple acid anhydride groups for linking to amine groups on biomolecules. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
-
4. The method of claim 4 where the oxide groups are selected from the group consisting of SiO2, Al2O3, TaO2 and TiO2.
-
18. A method for preparing a biosensor, comprising:
-
(a) providing a substrate having a surface reactive with a polyamine; (b) coating the substrate surface with the polyamine to provide a bonded polyamine; (c) forming multiple, physically discrete areas of exposed polyamine by patterning the substrate; and (d) linking a polyacid anhydride to the exposed bonded polyamine. - View Dependent Claims (19, 20)
-
-
21. A biosensor, comprising:
-
(a) a substrate; (b) a polyamine layer on the substrate; (c) a patterned layer on the polyamine layer which defines separate, discrete physical areas of exposed polyamine in the polyamine layer; (d) a polyanhydride layer on each discrete physical area of the exposed polyamine in the polyamine layer; and (e) a plurality of probe biomolecules attached to the polyanhydride layer through an amine group on the biomolecule, where the probe biomolecules are attached as different populations in each discrete physical area of the polyanhydride layer. - View Dependent Claims (22, 23, 24, 25)
-
Specification