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Heat and pH Measurement for Sequencing of DNA

  • US 20080166727A1
  • Filed: 12/18/2007
  • Published: 07/10/2008
  • Est. Priority Date: 12/20/2006
  • Status: Active Grant
First Claim
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1. A method for obtaining sequence information from a single stranded DNA template, comprising:

  • a) providing a primer region of the DNA template;

    b) placing multiple copies of the template DNA including the provided primer region in a reaction well, wherein the reaction well has a fluid volume of less than about 0.1 uL;

    c) adding to the reaction well a DNA polymerization mixture containing DNA polymerase and a plurality of nucleotides; and

    d) measuring a temperature change of at least 0.003°

    C., wherebyincorporation of nucleotides by the DNA polymerase produces heat increase indicative of incorporation of a complementary nucleotide into the DNA template.

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