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Microbial Growth Assay

  • US 20080166753A1
  • Filed: 03/02/2005
  • Published: 07/10/2008
  • Est. Priority Date: 04/12/2004
  • Status: Abandoned Application
First Claim
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1. A method of determining the susceptibility of a biofilm to an antimicrobial agent, comprising:

  • culturing microbes on a support to form a biofilm;

    contacting the biofilm with a metabolic substrate;

    determining a base-line metabolic activity of the biofilm by measuring a signal from the metabolic substrate;

    contacting the biofilm with one or more antimicrobial agents;

    determining an experimental metabolic activity by measuring a signal from the metabolic substrate; and

    comparing the base-line metabolic activity with the experimental metabolic activity, wherein a change is indicative of an antimicrobial agent that affects microbes in the biofilm.

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