Methods of increasing the concentration of a nucleic acid
0 Assignments
0 Petitions
Accused Products
Abstract
We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.
-
Citations
20 Claims
-
1. (canceled)
-
2. A method for increasing concentration of a nucleic acid molecule comprising the steps of:
-
(a) forming microcapsules from a thermostable water-in-oil emulsion, wherein a plurality of microcapsules include a nucleic acid molecule, a bead capable of being linked to the nucleic acid molecule, and an aqueous solution comprising components necessary to perform PCR nucleic acid amplification, including a thermostable polymerase, wherein the emulsion has a density of greater than 109 microcapsules per ml; (b) amplifying the nucleic acid molecule in the microcapsules to form amplified copies of the nucleic acid molecule;
thereby increasing the concentration of the nucleic acid molecule; and(c) sorting the bead with the captured amplification copies for sequencing. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification