Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
First Claim
1. An apparatus for identifying the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising:
- a droplet generator for creating droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing said organism sized particles;
a lysis device for performing lysis of the organisms to release the nucleic acids;
an amplifier for amplifying the nucleic acids;
a fractionater that releases the nucleic acids from said droplets; and
a parallel analyzer for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
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Accused Products
Abstract
A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
267 Citations
28 Claims
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1. An apparatus for identifying the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising:
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a droplet generator for creating droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing said organism sized particles; a lysis device for performing lysis of the organisms to release the nucleic acids; an amplifier for amplifying the nucleic acids; a fractionater that releases the nucleic acids from said droplets; and a parallel analyzer for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An apparatus for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising:
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a chip, a microfluidic flow channel in said chip, a droplet generator in said microfluidic flow channel for creating droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing said organism sized particles; a lysis device in said microfluidic flow channel for performing lysis of the organisms to release the nucleic acids; an amplifier in said microfluidic flow channel for amplifying the nucleic acids; a fractionater in said microfluidic flow channel that releases the nucleic acids from said droplets; and a parallel analyzer connected to said microfluidic flow channel for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification