METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE
First Claim
1. A method for detecting whether at least one molecule of a first target nucleic acid is present in a first sample portion, said method comprising:
- loading a first sample portion into a first sample chamber, said first sample chamber comprising at least a portion of an inside of a first microcapillary device, said first sample portion comprising at least part of a sample, whereby if said first sample portion contains at least a single molecule of said target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample portion after a single round of amplification;
subjecting said first sample portion in said first sample chamber to at least a first amplification step; and
thendetermining whether said first sample portion contains at least one molecule of said target nucleic acid.
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Accused Products
Abstract
A method comprising loading a sample portion into a sample chamber inside a microcapillary device, subjecting the sample portion to at least a first amplification step, and then determining whether the first sample portion contains at least one molecule of a target nucleic acid. Also, a microfluidic device comprising a sample chamber inside a microcapillary device, and a sample portion and/or an amplification targeting reagent in the sample chamber. Also, a microfluidic assembly comprising a microcapillary device having a sample chamber containing a reagent which enables amplification of a target nucleic acid. Also, a microfluidic device comprising a microcapillary device having a sample retaining means which contains a sample portion and/or an amplification targeting reagent.
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Citations
282 Claims
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1. A method for detecting whether at least one molecule of a first target nucleic acid is present in a first sample portion, said method comprising:
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loading a first sample portion into a first sample chamber, said first sample chamber comprising at least a portion of an inside of a first microcapillary device, said first sample portion comprising at least part of a sample, whereby if said first sample portion contains at least a single molecule of said target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample portion after a single round of amplification; subjecting said first sample portion in said first sample chamber to at least a first amplification step; and
thendetermining whether said first sample portion contains at least one molecule of said target nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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53. A microfluidic device comprising:
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a first sample chamber; and a first sample portion, said first sample chamber comprising at least a portion of an inside of a first microcapillary device, said first sample portion being positioned in said first sample chamber, whereby if said first sample portion contains at least a single molecule of a target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample chamber after a single round of amplification. - View Dependent Claims (54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88)
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89. A microfluidic device comprising:
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a first sample chamber; and at least one amplification targeting reagent positioned in said first sample chamber, said first sample chamber comprising at least a portion of an inside of a first microcapillary device, whereby if a sample portion which contains at least a single molecule of said target nucleic acid is loaded in said first sample chamber, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample chamber after a single round of amplification. - View Dependent Claims (90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121)
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122. A method for detecting, for each of a plurality of sample portions, whether the sample portion contains at least one molecule of at least a first target nucleic acid, said method comprising:
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loading sample portions of at least a first sample into a plurality of respective sample chambers, each said sample chamber comprising at least a portion of an inside of a microcapillary device, whereby if a sample portion loaded in one of said respective sample chambers contains at least a single molecule of said first target nucleic acid, said sample portion would attain a detectable concentration of said first target nucleic acid within a portion of said sample portion after a single round of amplification; subjecting said sample portions to at least a first amplification step; and
thenfor each of a plurality of said sample portions, determining whether said sample portion contains at least one molecule of said first target nucleic acid. - View Dependent Claims (123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159)
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160. A microfluidic assembly, comprising:
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at least a first microcapillary device, said microcapillary device defining at least a first sample chamber; at least one reagent which enables amplification of a target nucleic acid, said at least one reagent being positioned within said sample chamber. - View Dependent Claims (161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191)
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192. A method for detecting whether at least one molecule of a first target nucleic acid is present in a first sample portion, said method comprising:
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loading a first sample into a first region of a sample retaining means positioned within a microcapillary device, said first sample comprising at least part of a fluid sample, whereby if a first portion of said first sample contains at least a single molecule of said target nucleic acid, said first portion of said first sample would attain a detectable concentration of said target nucleic acid within a first portion of said sample retaining means after a single round of amplification; subjecting said first portion of said first sample to at least a first amplification step; and
thendetermining whether said first portion of said first sample contains at least one molecule of said target nucleic acid. - View Dependent Claims (193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234)
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235. A microfluidic device comprising:
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at least a first microcapillary device comprising at least a first microcapillary region; at least a first sample retaining means positioned within said first microcapillary region; and at least a first sample portion, said first sample portion being positioned in at least a first region of said first sample retaining means, whereby if said first sample portion contains at least a single molecule of a target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid after a single round of amplification. - View Dependent Claims (236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262)
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263. A microfluidic device comprising:
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at least a first microcapillary device comprising at least a first microcapillary region; at least a first sample retaining means positioned within said first microcapillary region; and at least one amplification targeting reagent positioned in at least a first region of said first sample retaining means, whereby if a sample portion which contains at least a single molecule of a target nucleic acid is loaded in said first region of said first sample retaining means, said sample portion would attain a detectable concentration of said target nucleic acid after a single round of amplification. - View Dependent Claims (264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282)
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Specification