METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE
First Claim
1. A method for detecting whether at least one molecule of a target nucleic acid is present in a first sample portion, said method comprising:
- depositing at least a first sample portion in a sample retaining means, said first sample portion comprising at least a portion of a first sample;
forcing a curable fluid across an exposed surface of said sample retaining means, thereby displacing any excess first sample from said exposed surface without displacing said sample from said sample retaining means.
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Accused Products
Abstract
A method comprising depositing a sample portion in a sample retaining means, and forcing a curable fluid across an exposed surface of the sample retaining means, thereby displacing excess first sample from the exposed surface without displacing the sample from the sample retaining means. Also, a method comprising depositing a sample portion in a sample retaining means, removing excess sample from an exposed surface of the sample retaining means, and depositing a curable fluid on the exposed surface. Also, a microfluidic device comprising a sample chamber, a first sample portion in the sample chamber, and a curable fluid and/or a cured fluid positioned on an exposed surface of a sample retaining means.
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Citations
20 Claims
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1. A method for detecting whether at least one molecule of a target nucleic acid is present in a first sample portion, said method comprising:
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depositing at least a first sample portion in a sample retaining means, said first sample portion comprising at least a portion of a first sample; forcing a curable fluid across an exposed surface of said sample retaining means, thereby displacing any excess first sample from said exposed surface without displacing said sample from said sample retaining means. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for detecting whether at least one molecule of a target nucleic acid is present in a first sample portion, said method comprising:
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depositing at least a first sample portion in a sample retaining means, said first sample portion comprising at least a portion of a first sample; removing excess first sample from said exposed surface; and depositing at least a first curable fluid on said exposed surface of said sample retaining means. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A microfluidic device comprising:
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a first sample chamber; and a first sample portion, said first sample portion being positioned in said first sample chamber; and a curable fluid positioned on an exposed surface of said sample retaining means, said curable fluid covering at least said first sample portion. - View Dependent Claims (14, 15, 16)
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17. A microfluidic device comprising:
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a first sample chamber; and a first sample portion, said first sample portion being positioned in said first sample chamber; and a cured fluid positioned on an exposed surface of said sample retaining means, said cured fluid covering at least said first sample portion. - View Dependent Claims (18, 19, 20)
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Specification