MICROFLUIDIC ASSEMBLY WITH REAGENT

• US 20080171380A1
• Filed: 08/13/2007
• Published: 07/17/2008
• Est. Priority Date: 04/17/1997
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated), United States Department Of Health And Human Services
• Original Assignee: Cytomix, The United States of America As Represented By The Secretary of Agriculture
• Inventors: Silver, Jonathan E., BROWN, James F.
• Application Number:
  US11/837,561
• Publication Number:
  US 20080171380A1
• Time in Patent Office: Days
• US Class Current: 435/283.1
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/06 Fluid handling related problems
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6844 : Nucleic acid amplification reactions
• CPC Class Codes: C12Q 1/686 : Polymerase chain reaction [PCR]
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A microfluidic assembly, comprises a first region having a first affinity to a first fluid sample and a second region having a second affinity to the first fluid sample. The first affinity is greater than the second affinity. A first reagent is positioned within at least the first region, the first reagent enabling amplification of a first target nucleic acid.

Claims

1. A microfluidic assembly, comprising:

at least a first region having a first affinity to a first fluid sample; and

at least a second region having a second affinity to said first fluid sample,

said first affinity being greater than said second affinity,

at least a first reagent being positioned within at least said first region, said first reagent enabling amplification of a first target nucleic acid.

2. A microfluidic assembly as recited in claim 1, wherein said microfluidic assembly further comprises at least a third region, said third region having a third affinity to said first fluid sample, said third affinity being greater than said second affinity, and

at least a second reagent being positioned within said third region, said second reagent enabling amplification of a second target nucleic acid.