METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE
First Claim
1. A microfluidic device comprising:
- a first sample chamber; and
a first sample portion, said first sample portion being positioned in said first sample chamber, whereby if said first sample portion contains at least a single molecule of a target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample chamber after a single round of amplification.
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Accused Products
Abstract
A microfluidic device comprising a first sample chamber and a first sample portion positioned in the first sample chamber. If the first sample portion contains a single molecule of a target nucleic acid, the first sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a first sample chamber and at least one amplification targeting reagent positioned in the first sample chamber. If a sample portion which comprises at least a single molecule of a target nucleic acid is positioned in the first sample chamber, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification.
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Citations
105 Claims
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1. A microfluidic device comprising:
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a first sample chamber; and a first sample portion, said first sample portion being positioned in said first sample chamber, whereby if said first sample portion contains at least a single molecule of a target nucleic acid, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample chamber after a single round of amplification. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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54. A microfluidic device comprising:
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a first sample chamber; and at least one amplification targeting reagent positioned in said first sample chamber, whereby if a sample portion which comprises at least a single molecule of a target nucleic acid is positioned in said first sample chamber, said first sample portion would attain a detectable concentration of said target nucleic acid within a portion of said first sample chamber after a single round of amplification. - View Dependent Claims (55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105)
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Specification