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Methods and means for altering fiber characteristics in fiber-producing plants

  • US 20080171391A1
  • Filed: 07/05/2007
  • Published: 07/17/2008
  • Est. Priority Date: 08/15/2003
  • Status: Active Grant
First Claim
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1. A method for increasing the length of a fiber in cotton, comprising introducing a the step of introducing a chimeric gene into a cell of said fiber producing plant, wherein said chimeric gene, when expressed in said cell of said fiber-producing plant increases said fiber elongation phase and increases said deposition of callose, said chimeric gene comprising(a) a plant expressible promoter;

  • (b) a transcribed DNA region, which when transcribed yields a double-stranded RNA molecule capable of reducing the expression of a gene endogenous to said fiber producing plant, said gene being involved in callose removal from said plasmodesmata, and said RNA molecule comprising a first and second RNA region whereini) said first RNA region comprises a nucleotide sequence of at least 19 consecutive nucleotides having at least about 94% sequence identity to the nucleotide sequence of SEQ ID No 1;

    ii) said second RNA region comprises a nucleotide sequence complementary to said 19 consecutive nucleotides of said first RNA region;

    iii) said first and second RNA region are capable of base-pairing to form a double stranded RNA molecule between at least said 19 consecutive nucleotides of said first and second region; and

    (c) a 3′

    end region comprising transcription termination and polyadenylation signals functioning in cells of said plant;

    orsaid chimeric gene comprising the following operably linked DNA elements;

    i) a plant-expressible promoter, preferably a plant-expressible promoter which controls transcription preferentially in said fiber cells;

    ii) a DNA region encoding a β

    -1,3 glucanase protein comprising the amino acid sequence of SEQ ID No 3 or the nucleotide sequence of SEQ ID No 2; and

    iii) a 3′

    end region comprising transcription termination and polyadenylation signals functioning in cells of said plant.

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