Oligonucleotide mediated nucleic acid recombination

• US 20080171668A1
• Filed: 11/30/2007
• Published: 07/17/2008
• Est. Priority Date: 01/19/1999
• Status: Active Grant

• Current Assignee: Codexis, Inc.
• Original Assignee: Maxygen Inc.
• Inventors: Patten, Phillip A., Ness, Jon E., Gustafsson, Claes, Minshull, Jeremy, Stemmer, Willem P.C., Bass, Steven H., Crameri, Andreas, Welch, Mark
• Granted Patent: US 8,029,988 B2
• Time in Patent Office: Days
• US Class Current: 506/23
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

Claims

1-104. -104. (canceled)

105. A method of producing a library of chimeric nucleic acid molecules encoding full-length chimeric proteins, said method comprising:

(a) synthesizing a plurality of ligatable synthetic oligonucleotides corresponding to parental nucleic acid sequences encoding full-length proteins; and

(b) assembling the oligonucleotides in order by ligation to produce the library of chimeric nucleic acid molecules encoding the full-length chimeric proteins.