Cardiovascular tissue culture substrate

• US 20080176206A1
• Filed: 06/27/2007
• Published: 07/24/2008
• Est. Priority Date: 01/18/2007
• Status: Abandoned Application

• Current Assignee: Gunze Limited
• Original Assignee: Gunze Limited
• Inventors: Ikada, Yoshito, Matsuda, Shojiro, Shinoka, Toshiharu, Sakamoto, Yuki, Matsumura, Goki, Taira, Tsuguyoshi
• Application Number:
  US11/769,080
• Publication Number:
  US 20080176206A1
• Time in Patent Office: Days
• US Class Current: 435/1.1
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

The substrate for culturing a cardiovascular tissue of the present invention is a substrate for culturing a cardiovascular tissue, which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, the foam comprising lactide (D, L, DL isomer)-ε-caprolactone copolymer containing lactide (D, L, DL isomer) in a content of 50 to 54 mole % and ε-caprolactone in a content of 50 to 46 mole %, and the reinforcing material being covered with the foam.

Claims

1. A substrate for culturing a cardiovascular tissue,

which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,

the foam comprising lactide (D, L, DL isomer)-ε

-caprolactone copolymer containing lactide (D, L, DL isomer) in a content of 50 to 54 mole % and ε

-caprolactone in a content of 50 to 46 mole %,

and the reinforcing material being covered with the foam.

9. A method of producing a cardiovascular tissue for transplantation, wherein a substrate surface is covered with a cell,

which comprises seeding a cell in vitro on the substrate for culturing a cardiovascular tissue according to claim 1, 2, 3, 4, 5, 6, 7 or 8, and further culturing the cell in vitro.

10. A method of regenerating a cardiovascular tissue,

which comprises seeding a cell in vitro on the substrate for culturing a cardiovascular tissue according to claim 1, 2, 3, 4, 5, 6, 7 or 8, and further culturing the cell to regenerate a cardiovascular tissue in vitro.

11. A cardiovascular tissue for transplantation,

which is obtained by seeding an endothelial cell, a marrow cell, a smooth muscle cell or a fibroblast in vitro on the substrate for culturing a cardiovascular tissue according to claim 1, 2, 3, 4, 5, 6, 7 or 8, and further culturing them in vitro.

2. A substrate for culturing a cardiovascular tissue,

which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,

the foam having a thickness of 0.2 to 3.0 mm,

the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.

3. A substrate for culturing a cardiovascular tissue,

which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,

the reinforcing material comprising a bioabsorbable fiber coated with a bioabsorbable material,

the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.

4. The substrate for culturing a cardiovascular tissue according to claim 3,

wherein the reinforcing material comprises a polyglycolic acid fiber coated with lactide (D, L, DL isomer)-ε

-caprolactone copolymer.

5. A substrate for culturing a cardiovascular tissue,

which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,

the reinforcing material comprising a twisted yarn comprising a twisted bioabsorbable multifilament yarn,

the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.

6. A substrate for culturing a cardiovascular tissue,

which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, and a reinforcing yarn comprising a bioabsorbable material,

the reinforcing yarn and the reinforcing material being situated at a center or on an outer plane of the foam, and an inner plane comprising the foam.

7. The substrate for culturing a cardiovascular tissue according to claim 6,

wherein the reinforcing yarn comprises at least one kind of compound selected from the group consisting of poly-L-lactide, lactide (D, L, DL isomer)-ε

-caprolactone copolymer and glycolic acid-ε

-caprolactone copolymer.

8. The substrate for culturing a cardiovascular tissue according to claim 6,

wherein the reinforcing yarn is wound in a spiral form, a ring form, or a X-shaped form.