Compositions, organisms and methodologies employing a novel human protein phosphatase

• US 20080178307A1
• Filed: 04/16/2007
• Published: 07/24/2008
• Est. Priority Date: 10/24/2002
• Status: Abandoned Application

• Current Assignee: Wyeth (Pfizer Inc.)
• Original Assignee: Wyeth (Pfizer Inc.)
• Inventors: Be, Xiaobing, Ford, Roger, Liu, Wei, Wu, Leeying
• Application Number:
  US11/787,345
• Publication Number:
  US 20080178307A1
• Time in Patent Office: Days
• US Class Current: 800/13
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)

Abstract

This invention provides compositions, organisms and methodologies employing a novel human gene encoding a protein that has sequence homology to a consensus sequence of calcineurin-like phosphoesterase family are disclosed. The novel protein is encoded by a human gene comprising 4 exons. The human gene is localized in the 10p15 locus of human chromosome 10. The sequence similarities between the novel human protein and the consensus sequence of calcineurin-like phosphoesterases indicate that the novel human protein may function as a calcineurin-like protein phosphatase.

Claims

1. An isolated polynucleotide comprising a nucleic acid sequence which encodes a polypeptide containing the amino acid sequence depicted in SEQ ID NO:

2 and having a calcineurin-like phosphoesterase function.

2. The polynucleotide according to claim 1, wherein the nucleic acid sequence is selected from the group consisting of:

(a) the nucleic acid sequence as shown in SEQ ID NO;

1;

(b) the complement of (a); and

(c) a nucleic acid sequence that differs from (a) or (b) due to the degeneracy of the genetic code.

3. The polynucleotide according to claim 1, wherein the nucleic acid sequence is selected from the group consisting of:

(a) the nucleic acid sequence as shown in SEQ ID NO;

3;

(b) the complement of (a); and

(c) a nucleic acid sequence that differs from (a) or (b) due to the degeneracy of the genetic code.

14. A host cell containing the polynucleotide of claim 1 or a variant thereof.

15. A transgenic non-human animal comprising the polynucleotide of claim 1 or a variant thereof.

4. An isolated polynucleotide comprising a variant of a nucleic acid sequence, wherein said nucleic acid sequence encodes the amino acid sequence depicted in SEQ ID NO:

2, and wherein the variant and said nucleic acid sequence have at least 90% sequence identity.

5. The polynucleotide according to claim 4, wherein the variant and said nucleic acid sequence have at least 95% sequence identity.

6. An isolated polynucleotide that is capable of hybridizing under stringent conditions to the nucleotide sequence of SEQ ID NO:

1 or the complement thereof.

7. The polynucleotide of claim 6, wherein the polynucleotide encodes a calcineurin-like phosphoesterase.

8-13. -13. (canceled)

16-23. -23. (canceled)