Fluidic methods

• US 20080178692A1
• Filed: 04/30/2007
• Published: 07/31/2008
• Est. Priority Date: 01/29/2007
• Status: Abandoned Application

• Current Assignee: Gearbox LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Lord, Robert W., Levien, Royce A., Jung, Edward K. Y., Malamud, Mark A., Leuthardt, Eric C., Rinaldo, John D., Wood, Lowell L.
• Application Number:
  US11/799,462
• Publication Number:
  US 20080178692A1
• Time in Patent Office: Days
• US Class Current: 73/863.21
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows

Abstract

The present disclosure relates to methods and devices that may be used to separate components from one or more samples.

Claims

1. A method comprising:

placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids; and

translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids.

3. The method of claim 1, wherein the placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

placing the one or more sample fluids that include one or more bodily samples into the one or more separation channels.

5. The method of claim 1, wherein the placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

placing the one or more sample fluids that include blood into the one or more separation channels.

7. The method of claim 1, wherein the placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

placing the one or more sample fluids that include one or more food samples into the one or more separation channels.

8. The method of claim 1, wherein the placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

placing the one or more sample fluids into the one or more separation channels so that the one or more sample fluids are in substantially parallel laminar flow with the one or more magnetically active fluids.

9. The method of claim 1, wherein the placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

placing the one or more sample fluids into the one or more separation channels so that the one or more sample fluids are in substantially anti-parallel laminar flow with the one or more magnetically active fluids.

14. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents through use of one or more ferrofluids.

15. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents through use of the one or more magnetically active fluids that include magnetic particles.

16. The method of claim 1, further comprising:

mixing one or more magnetically active agents with the one or more sample fluids to form the one or more magnetically active constituents.

18. The method of claim 1, further comprising:

detecting one or more constituents of the one or more sample fluids.

70. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents that include one or more non-ferrous tags.

71. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents that include one or more ferrous tags.

72. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents that include one or more magnetic tags.

73. The method of claim 1, wherein the translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

translocating the one or more magnetically active constituents that include one or more paramagnetic tags.

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74. A system comprising:

means for placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids; and

means for translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids.

75. The system of claim 74, further comprising:

means for mixing one or more magnetically active agents with one or more sample fluids to form one or more magnetically active constituents.

76. The system of claim 74, further comprising:

means for detecting one or more constituents of the one or more sample fluids.

77. A system comprising:

circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids; and

circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids.

78. The system of claim 77, wherein the circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

circuitry for facilitating placement of the one or more sample fluids that include one or more bodily samples into the one or more separation channels.

79. The system of claim 77, wherein the circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

circuitry for facilitating placement of the one or more sample fluids that include blood into the one or more separation channels.

80. The system of claim 77, wherein the circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

circuitry for facilitating placement of the one or more sample fluids that include one or more food samples into the one or more separation channels.

81. The system of claim 77, wherein the circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

circuitry for facilitating placement of the one or more sample fluids into the one or more separation channels so that the one or more sample fluids are in substantially parallel laminar flow with the one or more magnetically active fluids.

82. The system of claim 77, wherein the circuitry for facilitating placement of one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids comprises:

circuitry for facilitating placement of the one or more sample fluids into the one or more separation channels so that the one or more sample fluids are in substantially anti-parallel laminar flow with the one or more magnetically active fluids.

83. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents that include one or more non-ferrous tags.

84. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents that include one or more ferrous tags.

85. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents that include one or more magnetic tags.

86. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents that include one or more paramagnetic tags.

87. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents through use of one or more ferrofluids.

88. The system of claim 77, wherein the circuitry for facilitating translocation of one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids comprises:

circuitry for facilitating translocation of the one or more magnetically active constituents through use of the one or more magnetically active fluids that include magnetic particles.

89. The system of claim 77, further comprising:

circuitry for facilitating mixing of one or more magnetically active agents with one or more sample fluids to form one or more magnetically active constituents.

90. The system of claim 77, further comprising:

circuitry for facilitating detection of one or more constituents of the one or more sample fluids.

91. The system of claim 90, wherein the circuitry for facilitating detection of one or more constituents of the one or more sample fluids comprises:

circuitry for facilitating detection of the one or more constituents with one or more techniques that include spectroscopy, electrochemical detection, polynucleotide detection, fluorescence anisotropy, fluorescence resonance energy transfer, electron transfer, enzyme assay, magnetism, electrical conductivity, isoelectric focusing, chromatography, immunoprecipitation, immunoseparation, aptamer binding, electrophoresis, use of a CCD camera, or immunoassay.

92. A system comprising:

A signal-bearing medium bearing;

one or more instructions for placing one or more sample fluids into one or more separation channels so that the one or more sample fluids are in substantially laminar flow with one or more magnetically active fluids; and

one or more instructions for translocating one or more magnetically active constituents from the one or more sample fluids into the one or more magnetically active fluids.

93. The system of claim 92, further comprising:

one or more instructions for mixing one or more magnetically active agents with the one or more sample fluids to form the one or more magnetically active constituents.

94. The system of claim 92, further comprising:

one or more instructions for detecting one or more constituents of the one or more sample fluids.