Apparatus and Method for Interleaving Detection of Fluorescence and Luminescence
First Claim
1. An optical sandwich comprising:
- (i) a light source;
(ii) a high numerical aperture collimation lens;
(iii) an excitation filter selected from the group consisting of a sharp spectral band pass, a low pass filter and a high pass filter; and
(iv) a diffuser,wherein the optical elements (i), (ii), (iii) and (iv) are arranged to provide a spectrally pure and spatially dispersed illumination.
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Accused Products
Abstract
An apparatus is provided that is capable of interleaving detection of fluorescence and luminescence signals emitted from a plurality of samples. The apparatus is suitable for analysis of samples containing single cells or tissues up to and including living organisms. It contains an optical assembly or “sandwich” for producing a spectrally pure and spatially disperse light source for illuminating the sample. The invention also provides a plurality of optical sandwiches that can be variously geometrically arranged and their intensities programmed to create spatially uniform illumination over a large sample. The invention further provides an apparatus having at least one of the optical sandwich and a detector system capable of interleaving detection of fluorescent and luminescent signals when a suitable sample is illuminated by the light source of the optical sandwich. Methods for preparing samples and using the sandwiches, arrays and apparatus, are further provided by this invention. A method for interleaving detection of fluorescent and luminescent signals emitted from a plurality of samples is disclosed.
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Citations
45 Claims
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1. An optical sandwich comprising:
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(i) a light source; (ii) a high numerical aperture collimation lens; (iii) an excitation filter selected from the group consisting of a sharp spectral band pass, a low pass filter and a high pass filter; and (iv) a diffuser, wherein the optical elements (i), (ii), (iii) and (iv) are arranged to provide a spectrally pure and spatially dispersed illumination. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. A method for interleaving detection of fluorescent and luminescent signals emitted from a plurality of samples, comprising detecting fluorescent and luminescent signals produced by the plurality of samples, wherein the fluorescence excitation light source is automatically turned off or shuttered during interleaved luminescence imaging.
Specification