SP16 protein

• US 20080181895A1
• Filed: 11/06/2007
• Published: 07/31/2008
• Est. Priority Date: 11/07/1997
• Status: Abandoned Application

• Current Assignee: Incyte Corporation
• Original Assignee: Incyte Corporation
• Inventors: Mathur, Preete, Reddy, Roopa, Au-Young, Janice, Lal, Preeti G., Murry, Lynn E.
• Application Number:
  US11/979,577
• Publication Number:
  US 20080181895A1
• Time in Patent Office: Days
• US Class Current: 424/138.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/00 Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
• CPC Class Codes: C07K 14/435 from animals; from humans
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/036 targeting to the medium outside of the cell, e.g. type III secretion
• CPC Class Codes: C07K 2319/60 containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
• CPC Class Codes: C07K 2319/61 containing an enzyme fusion for detection (lacZ, luciferase)
• CPC Class Codes: C07K 2319/71 containing domain for transcriptional activaation, e.g. VP16
• CPC Class Codes: C07K 2319/80 containing a DNA binding domain, e.g. Lacl or Tet-repressor
• CPC Class Codes: C12N 15/625 containing a sequence coding for a signal sequence
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: G01N 33/574 for cancer
• CPC Class Codes: G01N 33/57415 of breast
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites

Abstract

The invention provides cDNAs which encodes a signal peptide-containing proteins. It also provides for the use of a cDNA, protein, and antibody in the diagnosis, prognosis, treatment and evaluation of therapies for cancer. The invention further provides vectors and host cells for the production of the protein and transgenic model systems.

Claims

1. An isolated antibody which specifically binds to a protein comprising an amino acid sequence selected from:

a) the amino acid sequence of SEQ ID NO;

16, and

b) an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO;

16 and having g-protein coupled receptor activity.

2. The isolated antibody of claim 1, wherein the protein comprises the amino acid sequence of SEQ ID NO:

16.

3. The isolated antibody of claim 1, wherein the protein comprises an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:

16 and having g-protein coupled receptor activity.

4. The isolated antibody of claim 1, wherein the antibody is a monoclonal antibody.

5. The isolated antibody of claim 1, wherein the antibody is a polyclonal antibody.

10. A method for using an antibody to detect expression of a protein in a sample, the method comprising:

a) combining the antibody of claim 1 with a sample under conditions which allow the formation of antibody;

protein complexes; and

b) detecting complex formation, wherein complex formation indicates expression of the protein in the sample.

12. A composition comprising an antibody of claim 1 and a therapeutic agent.

13. An array containing the antibody of claim 1.

6. A method of using a protein to prepare and purify a polyclonal antibody comprising:

a) immunizing a animal with a protein under conditions to elicit an antibody response, wherein the protein comprises an amino acid sequence selected from (i) the amino acid sequence of SEQ ID NO;

16, and (ii) an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO;

16 and having g-protein coupled receptor activity;

b) isolating animal antibodies;

c) attaching the protein to a substrate;

d) contacting the substrate with isolated antibodies under conditions to allow specific binding to the protein;

e) dissociating the antibodies from the protein, thereby obtaining purified polyclonal antibodies.

8. A polyclonal antibody produced by the method of claim 6.

7. A method of using a protein to prepare a monoclonal antibody comprising:

a) immunizing a animal with a protein under conditions to elicit an antibody response, wherein the protein comprises an amino acid sequence selected from (i) the amino acid sequence of SEQ ID NO;

16, and (ii) an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO;

16 and having g-protein coupled receptor activity;

b) isolating antibody producing cells from the animal;

c) fusing the antibody producing cells with immortalized cells in culture to form monoclonal antibody producing hybridoma cells;

d) culturing the hybridoma cells; and

e) isolating from culture monoclonal antibodies which specifically bind the protein.

9. A monoclonal antibody produced by the method of claim 7.

11. The method of claim 11, wherein complex formation is compared with standards and is diagnostic of cancer.