Methods and Compositions Involving MicroRNA
First Claim
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1. A method for multi-labeling miRNA in a sample comprising:
- a) enriching miRNA with respect to total RNA in the sample;
b) forming a reaction mixture, under conditions that allows enzyme catalysis, comprising the miRNA with i) an enzyme that catalyzes the addition of di- or tri-phosphate nucleotides to the 3′
end of the miRNA; and
ii) one or more labeled or unlabeled nucleotides, wherein tailed miRNA molecules are produced;
c) if unlabeled nucleotides are added to the miRNA, the method further comprising attaching a label to the tailed miRNA molecules.
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Abstract
The present invention concerns methods and compositions for isolating, enriching, and/or labeling miRNA molecules and for preparing and using arrays or other detection techniques for miRNA analysis. Moreover, the present invention concerns methods and compositions for generating miRNA profiles and employing such profiles for therapeutic, diagnostic, and prognostic applications.
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Citations
21 Claims
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1. A method for multi-labeling miRNA in a sample comprising:
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a) enriching miRNA with respect to total RNA in the sample; b) forming a reaction mixture, under conditions that allows enzyme catalysis, comprising the miRNA with i) an enzyme that catalyzes the addition of di- or tri-phosphate nucleotides to the 3′
end of the miRNA; and
ii) one or more labeled or unlabeled nucleotides, wherein tailed miRNA molecules are produced;c) if unlabeled nucleotides are added to the miRNA, the method further comprising attaching a label to the tailed miRNA molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21-147. -147. (canceled)
Specification