IMMERSION SENSOR FOR MEASURING THE CONCENTRATION OF AN ANALYTE WITH THE HELP OF AN OXIDE
First Claim
1. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase immobilized in or on an immersion sensor, wherein said analyte diffuses from a surface of said sensor into said enzyme region via at least one channel which limits diffusion and contains water.
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Accused Products
Abstract
The invention relates to measuring the concentration of analytes with the aid of an oxidase in an immersion sensor situated in a fluid or in a matrix containing fluid. Oxygen diffuses into the enzyme layer from within, from a gas-filled space connected to the atmosphere and/or to an oxygen reservoir. This enables oxygen saturation of the oxidase in a low-oxygen or oxygen-free medium and/or at high analyte concentrations. The analyte diffuses into the enzyme layer in a channel or a number of channels which contain(s) water and limit(s) diffusion, wherein the channel/channels on the surface of the sensor is/are filled with a protein-impermeable, hydrophilic matrix. By increasing the channel cross-section on the surface of the sensor and/or by connecting the channel/channels to a protein-impermeable, porous, hydrophilic layer on the surface of the sensor, the effect of outer deposits on the diffusion resistance of the analyte is reduced.
11 Citations
23 Claims
- 1. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase immobilized in or on an immersion sensor, wherein said analyte diffuses from a surface of said sensor into said enzyme region via at least one channel which limits diffusion and contains water.
- 4. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase immobilized in or on an immersion sensor, wherein the oxygen needed for oxidizing the analyte diffuses from within, directly from a gas-filled space or via an oxygen-permeable membrane, into the enzyme region.
- 9. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein at least one space for gaseous oxygen is formed in said immersion sensor, said space bordering the enzyme region, directly or via an oxygen-permeable material, from within.
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16. -20. (canceled)
- 21. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific enzyme associated with an immersion sensor, wherein said analyte diffuses from a surface of said sensor into said enzyme via at least one channel.
Specification