Method and reagent system with non-regenerable enzyme-coenzyme complex

• US 20080182324A1
• Filed: 01/10/2008
• Published: 07/31/2008
• Est. Priority Date: 05/16/2002
• Status: Active Grant

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Hoenes, Joachim, Horn, Carina, Knappe, Wolfgang-Reinhold
• Granted Patent: US 7,951,581 B2
• Time in Patent Office: Days
• US Class Current: 435/287.9
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y02P 20/582 Recycling of unreacted starting or intermediate materials

Abstract

The present invention is generally directed towards a reagent system for detecting an analyte in a sample. The reagent system has a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and a support to receive the detection reagent.

Claims

1. :

A reagent system for detecting an analyte in a sample, comprising;

(a) a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and

(b) a support to receive the detection reagent.

2. :

The reagent system as claimed in claim 1, characterized in that an oxidoreductase is used as the enzyme, and a change in the coenzyme due to oxidation or reduction is detected.

3. :

The reagent system as claimed in claim 1, characterized in that a coenzyme is selected from nicotine derivatives is used.

4. :

The reagent system as claimed in claim 1, characterized in that the change in the coenzyme is detected by optical methods.

5. :

The reagent system as claimed in claim 1, characterized in that the enzyme-coenzyme complex is embedded in a gel matrix.

6. :

The reagent system as claimed in claim 1, characterized in that the gel matrix has a layer thickness of ≦

50 μ

m.

7. :

The reagent system as claimed in claim 1, characterized in that the support is at least partly optically transparent.

8. :

The reagent system as claimed in claim 1, characterized in that the support comprises an essentially planar structure.

9. :

The reagent system as claimed in claim 1, characterized in that the support comprises a plurality of different detection reagents.

10. :

The reagent system as claimed in claim 1, characterized in that an analyte in a body fluid is detected.

11. :

The reagent system as claimed in claim 1, characterized in that a determination of glucose in the blood is carried out.

12. :

The reagent system as claimed in claim 1, characterized in that the duration of the reaction of the analyte is ≦

5 s.