Method and system for evaluation of signals received from spatially modulated excitation and emission to accurately determine particle positions and distances
First Claim
1. A method for determining a position of a particle comprising:
- detecting a particle based on a spatial modulation;
recording a time modulated signal based on the detecting of the particle;
applying a correlation routine to the time modulated signal to generate a transformed signal;
applying a fit function to the transformed signal; and
,extracting the position of the particle based on the applying of the fit function.
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Accused Products
Abstract
A method is provided for extracting the position of a particle, e.g., a moving or stationary particle that is excited or is emitting light. The method includes, among other steps, detecting and recording a signal based on, for example, the movement of the particle, a correlation step to eliminate noise and to create a transformed signal, a matching or fitting step to match the transformed signal to a fit function and an extracting or determining step to determine the position of the particle from the fit function. In one form, at least two particle positions are detected so that the distance between the subject particles can be determined.
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Citations
25 Claims
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1. A method for determining a position of a particle comprising:
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detecting a particle based on a spatial modulation; recording a time modulated signal based on the detecting of the particle; applying a correlation routine to the time modulated signal to generate a transformed signal; applying a fit function to the transformed signal; and
,extracting the position of the particle based on the applying of the fit function. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method to determine a position of at least one particle comprising:
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detecting a particle based on a spatial modulation; recording a time modulated signal based on the detecting of the particle; calculating a sliding integral over time on the recorded signal; applying a fit function to the sliding integral to obtain an estimated position of the particle; calculating a correlation signal; calculating a derivative signal of the correlation signal; determining a maximum and a minimum of the derivative signal based on the estimated position of the particle; and
,determining a zero transition to determine a calculated position of the particle. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A system for determining a position of a particle comprising:
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means for detecting a particle; means for recording a signal based on the detecting; means for applying a correlation routine to the signal to generate a transformed signal; means for applying a fit function to the transformed signal; and
,means for extracting the position of the particle based on the applying of the fit function. - View Dependent Claims (21, 22, 23, 24)
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25. A system to determine a position of at least one particle comprising:
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means for detecting a particle; means for recording a signal based on the detecting; means for calculating a sliding integral over time on the recorded signal; means for applying a fit function to the sliding integral to obtain an estimated position of the particle; means for calculating a correlation signal; means for calculating a derivative signal of the correlation signal; means for determining a maximum and a minimum of the derivative signal based on the estimated position of the particle; and
,means for determining a zero transition to determine a calculated position of the particle.
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Specification