HPV Primers

• US 20080187919A1
• Filed: 09/05/2007
• Published: 08/07/2008
• Est. Priority Date: 09/05/2006
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Third Wave Technologies Incorporated (Hologic Incorporated)
• Inventors: Hudson, Angela, King, Joseph J.
• Granted Patent: US 8,080,643 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

The present invention provides methods, kits, and compositions related to human papillomavirus (HPV) primers, which may be used, for example, in nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays.

Claims

1. A composition comprising an isolated nucleic acid sequence, wherein said nucleic acid sequence is 60 bases in length or less, and wherein said nucleic acid sequence comprises a nucleotide sequence selected from the group consisting of:

SEQ ID NOs;

1-123 and 142-154.

2. The composition of claim 1, wherein said nucleic acid sequence consists of one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

3. The composition of claim 1, wherein said nucleic acid sequence is 40 bases in length or less.

4. The composition of claim 1, wherein said nucleic acid sequence is 30 bases in length or less.

5. The composition of claim 1, further comprising a second isolated nucleic acid sequence different from said isolated nucleic acid sequence, wherein said second nucleic acid sequence is 50 bases in length or less, and wherein said second nucleic acid sequence comprises one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

6. The composition of claim 1, further comprising at least 20 additional isolated nucleic acid sequences, wherein said additional nucleic acid sequences are 60 bases in length or less, and wherein said additional nucleic acid sequence each comprises one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

7. A kit comprising an isolated nucleic acid sequence, wherein said nucleic acid sequence is 60 bases in length or less, and wherein said nucleic acid sequence comprises a nucleotide sequence selected from the group consisting of:

SEQ ID NOs;

1-123 and 142-154.

8. The kit of claim 7, wherein said nucleic acid sequence consists of one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

9. The kit of claim 7, wherein said nucleic acid sequence is 40 bases in length or less.

10. The kit of claim 7, wherein said nucleic acid sequence is 30 bases in length or less.

11. The kit of claim 7, further comprising a second isolated nucleic acid sequence different from said isolated nucleic acid sequence, wherein said second nucleic acid sequence is 50 bases in length or less, and wherein said second nucleic acid sequence comprises one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

12. The kit of claim 7, further comprising at least 20 additional isolated nucleic acid sequences, wherein said additional nucleic acid sequences are 60 bases in length or less, and wherein said additional nucleic acid sequence each comprises one of the nucleotide sequences shown in SEQ ID NOs:

1-123 and 142-154.

13. A method for detecting at least one HPV sequence in a sample, comprising using at least one nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of:

SEQ ID NOs;

1-123 and 142-154.

14. The method of claim 13, wherein said detecting allows typing of said HPC sequence.

15. The method of claim 13, wherein said using comprises sequencing with said nucleic acid sequence.

16. The method of claim 13, wherein said using comprises amplifying said HPV sequence with said nucleic acid sequence.

17. The method of claim 13, wherein said nucleic acid sequence is 60 bases in length or less.