OPTICAL DETERMINATION OF PH AND GLUCOSE
First Claim
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1. A device for determining two or more analyte concentrations, comprising:
- an optical sensor, comprising an indicator system, comprising;
a fluorophore that exists in at least first and second different forms depending on a concentration of a first analyte, wherein the different forms can be distinguished based on their respective first and second emissions; and
a binding moiety that binds a second analyte, wherein the binding moiety is operably coupled to said fluorophore, and wherein binding of said second analyte by said binding moiety causes an optical change in the apparent concentration of said fluorophore related to a concentration of said second analyte;
wherein a ratio of said first and second emissions is independent of the concentration of said second analyte;
a light source; and
a detector.
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Abstract
Embodiments of the present invention are directed to an optical sensor capable of measuring two analytes simultaneously with a single indicator system. In preferred embodiments, the sensor comprises a fluorescent dye having acid and base forms that facilitate ratiometric pH sensing, wherein the dye is further associated with a glucose binding moiety and configured to generate a signal that varies in intensity with the concentration of glucose.
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Citations
21 Claims
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1. A device for determining two or more analyte concentrations, comprising:
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an optical sensor, comprising an indicator system, comprising; a fluorophore that exists in at least first and second different forms depending on a concentration of a first analyte, wherein the different forms can be distinguished based on their respective first and second emissions; and a binding moiety that binds a second analyte, wherein the binding moiety is operably coupled to said fluorophore, and wherein binding of said second analyte by said binding moiety causes an optical change in the apparent concentration of said fluorophore related to a concentration of said second analyte; wherein a ratio of said first and second emissions is independent of the concentration of said second analyte; a light source; and a detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21)
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19. A device for determining blood pH and glucose concentration, comprising:
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a sensor comprising an optical fiber sized for deployment intravascularly, said sensor further comprising; a water-insoluble polymer matrix, wherein said polymer matrix is permeable to glucose; a fluorescent dye associated with said polymer matrix, wherein the fluorescent dye exhibits at least first and second different forms depending on pH, wherein the different forms can be distinguished based on their respective first and second emissions; a quencher comprising an aromatic boronic acid substituted viologen, adapted to reversibly bind an amount of glucose related to the blood glucose concentration, wherein said quencher is associated with said polymer matrix and operably coupled to the fluorescent dye, and wherein the quencher is configured to modulate the light emitted by said fluorescent dye related to the amount of bound polyhydroxyl compound; at least one excitation light source; and an emission light detector.
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Specification