SYSTEM AND METHOD FOR DETECTING FLUORESCENCE IN MICROFLUIDIC CHIP
First Claim
1. A fluorescence detection system for detecting fluorescence in a microfluidic chip having at least a microchamber, comprising:
- a chip mount for placing the microfluidic chip thereon;
an optical device for emitting light on the microfluidic chip and detecting fluorescence which is generated from a fluorescent material existing in the microfluidic chip;
a carriage for transporting the chip mount or the optical device at a predetermined speed, so that the optical device emits the light on the microfluidic chip while scanning the microfluidic chip;
a measuring unit for measuring an optical signal detected by the optical device and converting the optical signal into a digital signal; and
a calculator for calculating the amount of the fluorescent material in the microfluidic chip based on a measured value.
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Abstract
A system and a method for detecting fluorescence in a microfluidic chip having a microchamber and measuring the amount of the fluorescence are disclosed. In the system and method, an optical device detects fluorescence generated by a fluorescent material. The position of the outer circumference of a microfluidic chip is identified based on the change of the amount of the detected fluorescence. Then, the position of a microchannel is calculated based on the position of the outer circumference of the microfluidic chip and the distance between the outer circumference and the microchamber. Hence, the amount of the fluorescence only generated from the microchamber of the microfluidic chip is automatically calculated. Further, a plurality of samples can be analyzed at the same time, thereby reducing analysis time and cost.
99 Citations
19 Claims
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1. A fluorescence detection system for detecting fluorescence in a microfluidic chip having at least a microchamber, comprising:
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a chip mount for placing the microfluidic chip thereon; an optical device for emitting light on the microfluidic chip and detecting fluorescence which is generated from a fluorescent material existing in the microfluidic chip; a carriage for transporting the chip mount or the optical device at a predetermined speed, so that the optical device emits the light on the microfluidic chip while scanning the microfluidic chip; a measuring unit for measuring an optical signal detected by the optical device and converting the optical signal into a digital signal; and a calculator for calculating the amount of the fluorescent material in the microfluidic chip based on a measured value. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for detecting fluorescence in a microfluidic chip having at least one microchamber, comprising steps of:
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injecting a sample and a fluorescent dye into the microchamber; emitting light from an optical device capable of detecting fluorescence on the microfluidic chip, while transporting the microfluidic chip or the optical device, to detect fluorescence generated from a fluorescent material existing in the microfluidic chip; and calculating the amount of the fluorescent material in the microfluidic chip based on the amount of the fluorescence detected by the optical device. - View Dependent Claims (18, 19)
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Specification