Quasirandom Structure and Function Guided Synthesis Methods
First Claim
1. A method for synthesising at least one molecule comprising the steps ofi) providing a plurality of connector polynucleotides each capable of hybridizing to at least 1 complementary connector polynucleotide,ii) providing a plurality of complementary connector polynucleotides selected from the group consisting ofa) complementary connector polynucleotides comprising at least 1 reactant, such as a functional entity comprising at least 1 reactive group,b) complementary connector polynucleotides comprising at least 1 reactive group,c) complementary connector polynucleotides comprising at least 1 spacer region,iii) hybridizing at least 2 complementary connector polynucleotides to at least 2 connector polynucleotides,wherein at least 2 of said complementary connector polynucleotides comprise at least 1 reactant, such as a functional entity comprising at least 1 reactive group, wherein at least 1 of said complementary connector polynucleotides hybridizes to at least 2 connector polynucleotides, andiv) reacting at least 2 reactants or functional entity reactive groups by reacting at least 1 reactive group of each reactant or functional entity, wherein the reaction of said reactants or functional entity reactive groups results in the formation of the molecule by reacting the reactive groups of the reactants provided by separate complementary connector polynucleotides, or by covalently linking at least 2 functional entities provided by separate complementary connector polynucleotides.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention is directed to the synthesis of molecules guided b connector polynucleotides (CPNs capable of hybridizing to complementary connector polynucleotides (CCPNs) harbouring at least one functional entity comprising at least one reactive group. At least one of said CCPNs capable of hybridize to at least two CPNs. Each CPN will “call” for one or more CCPNs capable of hybridization to the CPN. Following the formation of a supramolecular hybridization complex comprising a plurality of CPNs and a plurality of CCPNs, the reaction of functional entity reactive groups result I the formation of a molecule comprising covalently linked functional entities. The formation of the molecule involves the transfer of functional entities from one or more “donor CCPNs” to at least on “acceptor CCPN” with which the transferred functional entities were not associated prior to the transfer.
-
Citations
117 Claims
-
1. A method for synthesising at least one molecule comprising the steps of
i) providing a plurality of connector polynucleotides each capable of hybridizing to at least 1 complementary connector polynucleotide, ii) providing a plurality of complementary connector polynucleotides selected from the group consisting of a) complementary connector polynucleotides comprising at least 1 reactant, such as a functional entity comprising at least 1 reactive group, b) complementary connector polynucleotides comprising at least 1 reactive group, c) complementary connector polynucleotides comprising at least 1 spacer region, iii) hybridizing at least 2 complementary connector polynucleotides to at least 2 connector polynucleotides, wherein at least 2 of said complementary connector polynucleotides comprise at least 1 reactant, such as a functional entity comprising at least 1 reactive group, wherein at least 1 of said complementary connector polynucleotides hybridizes to at least 2 connector polynucleotides, and iv) reacting at least 2 reactants or functional entity reactive groups by reacting at least 1 reactive group of each reactant or functional entity, wherein the reaction of said reactants or functional entity reactive groups results in the formation of the molecule by reacting the reactive groups of the reactants provided by separate complementary connector polynucleotides, or by covalently linking at least 2 functional entities provided by separate complementary connector polynucleotides.
-
60. A method for synthesising a plurality of different molecules, said method comprising
i) providing a plurality of connector polynucleotides each capable of hybridizing to at least 1 complementary connector polynucleotide, ii) providing a plurality of complementary connector polynucleotides selected from the group consisting of a) complementary connector polynucleotides comprising at least 1 functional entity comprising at least 1 reactive group, b) complementary connector polynucleotides comprising at least 1 reactive group, c) complementary connector polynucleotides comprising at least 1 spacer region, iii) hybridizing the plurality of connector polynucleotides and complementary connector polynucleotides, thereby forming a plurality of different hybridisation complexes, each hybridisation complex comprising at least 2 complementary connector polynucleotides and at least 2 connector polynucleotides, wherein, for each of said hybridisation complexes, at least 2 of said complementary connector polynucleotides comprise at least 1 functional entity comprising at least 1 reactive group, and at least 1 of said complementary connector polynucleotides hybridizes to at least 2 connector polynucleotides, and iv) reacting at least 2 functional entity reactive groups of each complex by reacting at least 1 reactive group of each functional entity, wherein, for each hybridisation complex, the reaction of said functional entity reactive groups results in the formation of a different molecule by covalently linking at least 2 functional entities provided by separate complementary connector polynucleotides, thereby synthesising a plurality of different molecules.
-
108. A method for synthesising at least one molecule, said method comprising the steps of
i) providing a plurality of building block polynucleotides each capable of hybridizing to at least 1 other building block polynucleotide, wherein said building block polynucleotides are selected from the group consisting of a) building block polynucleotides comprising at least 1 reactant comprising at least 1 reactive group b) building block polynucleotides comprising at least 1 reactive group, c) building block polynucleotides comprising at least 1 spacer region, ii) forming a hybridization complex comprising at least 4 building block polynucleotides, wherein at least 2 of said building block polynucleotides comprise at least 1 reactant comprising at least 1 reactive group, wherein at least 1 of said building block polynucleotide hybridizes to at least 2 other building block polynucleotides, and iii) synthesising the at least one molecule by reacting at least 2 reactants.
-
115. A method for synthesising a plurality of different molecules, said method comprising the steps of
i) providing a plurality of at least 1000 different building block polynucleotides each comprising at least one reactant, ii) selecting or providing from said plurality of building block polynucleotides n different building block polynucleotides for the synthesis of each different molecule, wherein n is an integer of at least 3 and less than 200, iii) optionally further providing to the reaction compartment a plurality of building block polynucleotides selected from the group consisting of building block polynucleotides comprising at least 1 reactive group (type II) and building block polynucleotides comprising at least 1 spacer region and no functional entity or reactive group (type III), iv) hybridizing at least the selected or provided n building block polynucleotides to form a hybridization complex, wherein at least n of said building block polynucleotides comprise at least 1 reactant comprising at least 1 reactive group, wherein at least 1 of said building block polynucleotides hybridizes to at least 2 other building block polynucleotides, with the proviso that no single building block polynucleotide hybridizes to the remaining n-T building block polynucleotides, and v) reacting the at least n reactants by reacting at least 1 reactive group of each reactant, wherein the reaction of said reactants provided by separate building block polynucleotides results in the formation of at least one molecule, wherein the at least one molecule is linked to at least one building block polynucleotide by at least one linker, and repeating the steps ii) to v) for different selections or provisions of building block polynucleotides each comprising at least one reactant, thereby generating a plurality of different molecules.
Specification