Photoacoustic Assay Method and Apparatus
First Claim
1. Apparatus for assaying a target analyte in a localized tissue region that may include the target and other analytes comprising:
- a light source that illuminates the region with light at each of a plurality of wavelengths at which light is absorbed and/or scattered by tissue in the region wherein light at at least one of the wavelengths is absorbed or and/or scattered by the target analyte;
a signal generator that generates signals responsive to intensity of the light from the light source at different locations in the localized region; and
a controller that;
receives the generated signals;
processes the signals to determine an extinction coefficient for light in the localized region at each wavelength; and
determines the concentration of the target analyte responsive to a solution of a set of simultaneous equations having as unknown variables concentrations of a plurality of analytes in the region, one of which is the target analyte, wherein each equation in the set expresses a relationship between the extinction coefficient, the absorption coefficient and/or the reduced scattering coefficient for light at a different one of the plurality of wavelengths and at least one of the equations expresses a relationship between the extinction coefficient and the reduced scattering coefficient.
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Accused Products
Abstract
Apparatus (20, 100) for assaying a target analyte in a localized tissue region (22) that may include the target and other analytes comprising: a light source (34, 104) that illuminates the region with light at each of a plurality of wavelengths at which light is absorbed and/or scattered by tissue in the region wherein light at a least one of the wavelengths is absorbed or scattered by the target analyte; a signal generator (40) that generates signals responsive to intensity of the light from the light source (34, 104) at different locations in the localized region (22); and a controller (32, 102) that: receives the generated signals; processes the signals to determine an extinction coefficient for light in the localized region at each wavelength; and determines the concentration of the target analyte responsive to a solution of a set of simultaneous equations having as unknown variables concentrations of a plurality of analytes in the region (22), one of which is the target analyte, wherein each equation in the set expresses a relationship between the extinction coefficient, the absorption coefficient and/or the reduced scattering coefficient for light at a different one of the plurality of wavelengths and at least one of the equations expresses a relationship between the extinction coefficient and the reduced scattering coefficient.
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Citations
28 Claims
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1. Apparatus for assaying a target analyte in a localized tissue region that may include the target and other analytes comprising:
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a light source that illuminates the region with light at each of a plurality of wavelengths at which light is absorbed and/or scattered by tissue in the region wherein light at at least one of the wavelengths is absorbed or and/or scattered by the target analyte; a signal generator that generates signals responsive to intensity of the light from the light source at different locations in the localized region; and a controller that; receives the generated signals; processes the signals to determine an extinction coefficient for light in the localized region at each wavelength; and determines the concentration of the target analyte responsive to a solution of a set of simultaneous equations having as unknown variables concentrations of a plurality of analytes in the region, one of which is the target analyte, wherein each equation in the set expresses a relationship between the extinction coefficient, the absorption coefficient and/or the reduced scattering coefficient for light at a different one of the plurality of wavelengths and at least one of the equations expresses a relationship between the extinction coefficient and the reduced scattering coefficient. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of assaying a target analyte in a region of body tissue that may include the target and other analytes comprising:
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determining an extinction coefficient for light at each of a plurality of different wavelengths at which light is absorbed and/or scattered by tissue in the region and wherein light at least one of the wavelengths is absorbed and/or scattered by the analyte; providing a value or an analytic expression for the reduced scattering coefficient at each wavelength; and determining the concentration of the target analyte responsive to a solution of a set of simultaneous equations having as unknown variables concentrations of a plurality of analytes in the region, one of which is the target analyte, wherein each equation in the set expresses a relationship between the extinction coefficient, the absorption coefficient and/or the reduced scattering coefficient for light at a different one of the plurality of wavelengths and at least one of the equations expresses a relationship between the extinction coefficient and the reduced scattering coefficient. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification