Reagent Composition and Methods of Using and Forming the Same
First Claim
Patent Images
1. A reagent composition for making electrochemical biosensors comprising:
- (a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample;
(b) a hydrophilic polymer selected from the group consisting of cellulose derivatives, natural gums and gels, and water soluble synthetic polymers;
(c) a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays;
(d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5 to 6.0;
(e) a surfactant; and
(f) a mediator.
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Abstract
A reagent composition containing GDH-PQQ as an enzyme-co-factor and screen-printed on working and counter electrodes of electrochemical biosensors, maintains activity of the enzyme reagents by proper selection of components. A preferred composition includes hydrophilic polymers, amorphous untreated silica, buffers, surfactants, and a mediator For example, the biosensor is useful in the amperometric determination of glucose.
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Citations
32 Claims
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1. A reagent composition for making electrochemical biosensors comprising:
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(a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample; (b) a hydrophilic polymer selected from the group consisting of cellulose derivatives, natural gums and gels, and water soluble synthetic polymers; (c) a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays; (d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5 to 6.0; (e) a surfactant; and (f) a mediator. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 11, 12)
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8. (canceled)
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10. (canceled)
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13. (canceled)
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14. (canceled)
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15. (canceled)
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16. (canceled)
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17. (canceled)
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18. An electrochemical biosensor comprising:
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(a) a non-porous substrate; (b) working and counter electrodes disposed on said substrate; (c) a reagent composition located on said electrodes, said composition comprising; (1) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample, for each milligram of the total weight of said composition; (2) a hydrophilic polymer selected from the group consisting of cellulose derivatives, natural gums and gels, and water soluble synthetic polymers; (3) a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays; (4) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5, (5) a surfactant, and (6) a mediator; and (d) a protective cover for said electrodes and said reagent composition. - View Dependent Claims (19, 20, 21, 23, 24, 26, 27, 28)
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22. (canceled)
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25. (canceled)
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29. A method of placing a reagent composition in an electrochemical biosensor, the method comprising screen printing a reagent composition, the reagent composition including:
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(a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample; (b) a hydrophilic polymer selected from the group consisting of cellulose derivatives, natural gums and gels, and water soluble synthetic polymers; (c) a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays; (d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5; (e) a surfactant; and (f) a mediator. - View Dependent Claims (31, 32)
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30. (canceled)
Specification