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Method for Functionalizing Biosensor Chips

  • US 20080199974A1
  • Filed: 03/18/2005
  • Published: 08/21/2008
  • Est. Priority Date: 04/21/2004
  • Status: Active Grant
First Claim
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1. A method for functionalizing a biosensor, comprising:

  • providing a substrate, including least one sensor field arranged thereon;

    applying a photoresist layer over an entire surface of the substrate and then photolithographically patterning the photoresist layer using a photomask with a predetermined pattern, so that a layer which has been patterned in a predetermined way is formed, to form a corresponding cavity above the sensor field;

    applying capture molecules to the sensor field, so that the capture molecules are immobilized within the cavity above the sensor field,wherein prior to application of capture molecules, a carrier layer based on hydrogel is applied to the sensor fields, wherein the application of capture molecules to the sensor fields is carried out with simultaneous application of a carrier layer based on hydrogel to the sensor fields, and wherein the application of capture molecules is followed by the application of an encapsulation layer based on hydrogel; and

    mounting the wafer, functionalized in such a manner following the application of capture molecules upside-down mode to a sawing sheet, tensioned in a sawing frame, and sawing the individual chips out of the wafer.

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