Bead Bound Combinatorial Oligonucleoside Phosphorothioate And Phosphorodithioate Aptamer Libraries
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Abstract
The present invention includes composition and methods for making and using a combinatorial library having two or more beads, wherein attached to each bead is a unique nucleic acid aptamer that have disposed thereon a unique sequence. The library aptamers may be attached covalently to the one or more beads, which may be polystyrene beads. The aptamers may include phosphorothioate, phosphorodithioate and/or methylphosphonate linkages and may be single or double stranded DNA, RNA or even PNAs.
78 Citations
62 Claims
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1-30. -30. (canceled)
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30. A method for aptamer selection comprising the steps of:
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dispersing a one-aptamer, one-bead combinatorial bead library into a two-dimensional matrix; scanning for aptamer beads that generate a detectable signal from interaction between the one or more aptamer beads and a target; and picking one or more aptamer beads based on the detectable signal from within the matrix. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61)
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62-81. -81. (canceled)
Specification