HIGH THROUGHPUT METHOD TO IDENTIFY LIGANDS FOR CELL ATTACHMENT

• US 20080200345A1
• Filed: 01/16/2008
• Published: 08/21/2008
• Est. Priority Date: 09/15/2003
• Status: Abandoned Application

• Current Assignee: Andrea Liebmann-Vinson, Chris H. Bodily, Jonathan A. Rowley, Mohammad A. Heidaran
• Original Assignee: Andrea Liebmann-Vinson, Chris H. Bodily, Jonathan A. Rowley, Mohammad A. Heidaran
• Inventors: Heidaran, Mohammad A., Rowley, Jonathan A., Liebmann-Vinson, Andrea, Bodily, Chris H.
• Application Number:
  US12/015,233
• Publication Number:
  US 20080200345A1
• Time in Patent Office: Days
• US Class Current: 506/10
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity
• CPC Class Codes: G01N 33/502 for testing non-proliferative effects
• CPC Class Codes: G01N 33/5029 on cell motility
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.

Claims

1. A high throughput method for identifying agents capable of producing a desired biological response in whole cells, the method comprising:

(a) providing receptacles having a culture surface;

(b) placing different mixtures comprising single said agents into selective ones of said receptacles according to a statistical design that is a mixture design;

(c) immobilizing said mixtures of single agents to said culture surface;

(d) contacting said agents from (c) with said whole cells;

(e) acquiring data indicative of said desired biological response in said contacted cells; and

(f) identifying which of said mixtures of single agents are effective in producing said desired biological response in said contacted cells using statistical modeling of said acquired data.

2. The method of claim 1, further comprising the step of placing single said agents into others of said receptacles.

3. The method of claim 1, wherein said culture surface is coated with an agent-immobilizing material.

4. The method of claim 3, wherein said agent-immobilizing material contains reactive groups for covalently immobilizing said agents.

5. The method of claim 3, wherein said agent-immobilizing material on said culture surface does not support cell adhesion.

6. The method of claim 3, wherein said agent-immobilizing material is hyaluronic acid.

7. The method of claim 1, wherein said data is acquired by a functional assay.

8. The method of claim 1, wherein said desired biological response is cell differentiation.

9. The method of claim 1, wherein said receptacles are wells of a 96-well plate.

10. The method of claim 1, further comprising repeating said steps with a subset of said identified mixtures of single agents.

11. The method of claim 1, wherein said statistical modeling is an algorithm for comparing said acquired data with the statistical design.