AMINO ACID SUBSTITUTED MOLECULES
First Claim
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1. A method of modifying a polypeptide containing a non-natural amino acid, comprising incorporating a chemical moiety into said polypeptide by a chemical reaction selected from the group consisting of:
- palladium-catalyzed Suzuki coupling, palladium-catalyzed Sonogashira coupling, palladium-catalyzed Heck coupling, palladium-catalyzed Hiyama coupling and Huisgen copper-catalyzed [3+2]cycloaddition, in the presence of micelles or DTT, or both, wherein said modified polypeptide retains its native function.
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Abstract
The invention provides compositions and methods of identifying, modifying and producing modified target molecules, including therapeutic molecules by modification with non-natural amino acids. Certain aspects of the invention include methods of adding a chemical moiety to a target molecule, and the compositions resulting therefrom. Certain aspects of the invention also relate to kits for identifying, modifying and producing modified target molecules described herein.
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13 Claims
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1. A method of modifying a polypeptide containing a non-natural amino acid, comprising incorporating a chemical moiety into said polypeptide by a chemical reaction selected from the group consisting of:
- palladium-catalyzed Suzuki coupling, palladium-catalyzed Sonogashira coupling, palladium-catalyzed Heck coupling, palladium-catalyzed Hiyama coupling and Huisgen copper-catalyzed [3+2]cycloaddition, in the presence of micelles or DTT, or both, wherein said modified polypeptide retains its native function.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
Specification