Methods, Reagents, Devices and Instrumentation For Preparing Impregnated Tissue Samples Suitable For Histopathological and Molecular Studies

• US 20080206807A1
• Filed: 07/31/2006
• Published: 08/28/2008
• Est. Priority Date: 07/29/2005
• Status: Active Grant

• Current Assignee: Cellcarta NV
• Original Assignee: HistoGeneX N.V.
• Inventors: Kockx, Marc, Duymelinck, Carla
• Granted Patent: US 8,691,165 B2
• Time in Patent Office: Days
• US Class Current: 435/40.5
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/049 Valves integrated in closure
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples
• CPC Class Codes: G01N 2001/315 Basket-type carriers for tissues
• CPC Class Codes: G01N 2035/00782 reprogrammmable code
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A process for the production of paraffin sections of biological tissue, especially for molecular pathology studies is disclosed. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. A kit and a processing station for automating paraffin embedding of a tissue sample suitable for histopathological and molecular analysis is also described. A bio-indicator system is described for measuring the degree of crosslinking. A tissue sample holding means or a vial which includes a tissue sample holding means provided with a data logging device capable of registering and transmitting data regarding the sample and conditions where the sample was processed is also disclosed.

Claims

1. A system comprising:

a vial configured to receive and retain liquids therein, comprising a vial body and a resealable lid configured to fittingly and reversibly seal a top opening of the vial, and

a tissue sample cassette,

wherein the vial or the tissue sample cassette is provided with a data logging device capable of registering and transmitting data regarding a sample and conditions wherein said sample is processed, wherein said data logging device comprises;

means for monitoring time (t) during which said sample is processed,

optionally, means for monitoring temperature (T) of the sample in function of time (t), and

an on/off function adapted to activate/deactivate the data logging device, such that said monitoring takes place once the data logging device has been activated,

said system characterised in that the tissue sample cassette is connected to the resealable lid by means of an elongated member.

2. The system according to claim 1, wherein the elongated member is configured to position the tissue sample cassette within the lower half of the inner space of the vial body when the lid seals the top opening of the vial.

3. The system according to claim 1, wherein said data logging device is provided on the vial body or on the resealable lid.

4. The system according to claim 1, wherein said data logging device is provided on the elongated member.

5. The system according to claim 1, wherein said data logging device is provided on the tissue sample cassette, preferably in a cavity of said tissue sample cassette, more preferably embedded in a suitable matrix in the cavity of said tissue sample cassette.

6. The system according to claim 1, wherein said data logging device comprises means for monitoring time (t) during which said sample is processed and means for monitoring temperature (T) of the sample in function of time (t).

7. The system according to claim 1, wherein said on/off function is adapted to be activated manually or automatically.

8. The system according to claim 1, wherein said data logging device comprises a clock and/or timer.

9. The system according to claim 1, wherein said data logging device comprises a thermometer.

10. The system according to claim 1, wherein said data logging device further comprises a sensor capable of measuring electrical conductance.

11. The system according to claim 1, wherein said data logging device comprises an identification means capable of identifying said sample.

12. The system according to claim 1, wherein said data logging device comprises a memory storage component capable of storing said registered data.

13. The system according to claim 1, wherein said data logging device is connectable to a reading device which is adapted to receive, read and process the data registered by said data logging device.

14. The system according to claim 1, wherein the vial comprises at least one breakable seal towards the top of the vial, suitable for receiving or removing fluid there through.

15. The system according to claim 1, wherein the vial comprises at least one breakable seal towards the top of the vial, suitable for applying positive or negative air pressure to the vial there through.

16. The system according to claim 1, wherein the vial comprises at least one breakable seal towards the base of the vial, suitable for receiving fluid there through.

17. A method for processing a tissue sample with the system of claim 1 which comprises activating said data logging device substantially concurrently with:

(i) the deposition of the tissue sample to be processed into the tissue sample cassette of said system, or

(ii) the exposure of the tissue sample cassette comprising said tissue sample to a first treatment solution comprising a fixative, in the vial of said system.

18. The method according to claim 17, comprising:

(i) querying the data logging device to determine the elapsed time during which the tissue sample has been exposed to fixative,

(ii) comparing said elapsed time with a pre-decided cut-off value,

(iii) if the elapsed time is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the elapsed time is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.

19. The method according to claim 17, comprising:

(i) querying the data logging device to determine the integral sum of temperature as a function of time during which the tissue sample has been exposed to fixative,

(ii) comparing said integral sum with a pre-decided cut-off value,

(iii) if the integral sum is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the integral sum is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.

20. A method for processing of a tissue sample, comprising:

(a) providing a tissue sample cassette provided with a data logging device capable of registering and transmitting data regarding a sample and conditions wherein said sample is processed, wherein said data logging device comprises;

means for monitoring time (t) during which said sample is processed,

optionally, means for monitoring temperature (T) of the sample in function of time (t), and

an on/off function adapted to activate/deactivate the data logging device, such that said monitoring takes place once the data logging device has been activated;

(b) activating said data logging device substantially concurrently with;

(i) the deposition of the tissue sample to be processed into the tissue sample cassette, or

(ii) the exposure of the tissue sample cassette comprising said tissue sample to a first treatment solution comprising a fixative;

(c) querying the data logging device to determine the elapsed time during which the tissue sample has been exposed to fixative;

(d) comparing said elapsed time with a pre-decided cut-off value;

(e) if the elapsed time is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the elapsed time is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.

24. The method of claim 20, wherein said data logging device is provided on the tissue sample cassette.

21. A method for processing of a tissue sample, comprising:

(a) providing a tissue sample cassette provided with a data logging device capable of registering and transmitting data regarding a sample and conditions wherein said sample is processed, wherein said data logging device comprises;

means for monitoring time (t) during which said sample is processed,

means for monitoring temperature (T) of the sample in function of time (t), and

an on/off function adapted to activate/deactivate the data logging device, such that said monitoring takes place once the data logging device has been activated;

(b) activating said data logging device substantially concurrently with;

(i) the deposition of the tissue sample to be processed into the tissue sample cassette, or

(ii) the exposure of the tissue sample cassette comprising said tissue sample to a first treatment solution comprising a fixative;

(c) querying the data logging device to determine the integral sum of temperature as a function of time during which the tissue sample has been exposed to fixative;

(d) comparing said integral sum with a pre-decided cut-off value;

(e) if the integral sum is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the integral sum is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.

22. A method for processing of a tissue sample, comprising:

(a) providing a system comprising;

a vial configured to receive and retain liquids therein, comprising a vial body and a resealable lid configured to fittingly and reversibly seal a top opening of the vial, and

a tissue sample cassette,

wherein the vial or the tissue sample cassette is provided with a data logging device capable of registering and transmitting data regarding a sample and conditions wherein said sample is processed, wherein said data logging device comprises;

means for monitoring time (t) during which said sample is processed,

optionally, means for monitoring temperature (T) of the sample in function of time (t), and

an on/off function adapted to activate/deactivate the data logging device, such that said monitoring takes place once the data logging device has been activated;

(b) activating said data logging device substantially concurrently with;

(i) the deposition of the tissue sample to be processed into the tissue sample cassette of said system, or

(ii) the exposure of the tissue sample cassette comprising said tissue sample to a first treatment solution comprising a fixative, in the vial of said system;

(c) querying the data logging device to determine the elapsed time during which the tissue sample has been exposed to fixative;

(d) comparing said elapsed time with a pre-decided cut-off value;

(e) if the elapsed time is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the elapsed time is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.

23. A method for processing of a tissue sample, comprising:

(a) providing a system comprising;

a vial configured to receive and retain liquids therein, comprising a vial body and a resealable lid configured to fittingly and reversibly seal a top opening of the vial, and

a tissue sample cassette,

wherein the vial or the tissue sample cassette is provided with a data logging device capable of registering and transmitting data regarding a sample and conditions wherein said sample is processed, wherein said data logging device comprises;

means for monitoring time (t) during which said sample is processed,

means for monitoring temperature (T) of the sample in function of time (t), and

an on/off function adapted to activate/deactivate the data logging device, such that said monitoring takes place once the data logging device has been activated;

(b) activating said data logging device substantially concurrently with;

(i) the deposition of the tissue sample to be processed into the tissue sample cassette of said system, or

(ii) the exposure of the tissue sample cassette comprising said tissue sample to a first treatment solution comprising a fixative, in the vial of said system;

(c) querying the data logging device to determine the integral sum of temperature as a function of time during which the tissue sample has been exposed to fixative;

(d) comparing said integral sum with a pre-decided cut-off value;

(e) if the integral sum is greater than said cut-off value, subjecting the tissue sample to post-fixation tissue processing steps, or, if the integral sum is lower than said cut-off value, calculating the time needed to completely fix the tissue and continuing fixation for said time before subjecting the tissue sample to post-fixation tissue processing steps.