COMPOSITIONS AND METHODS FOR THE TARGETED INSERTION OF A NUCLEOTIDE SEQUENCE OF INTEREST INTO THE GENOME OF A PLANT
First Claim
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1. A method for targeting the insertion of a polynucleotide of interest to a specific chromosomal site in a genome of a plant cell, said method comprising:
- a) introducing into the plant cell a transfer cassette by particle bombardment, said transfer cassette comprising said polynucleotide of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are non-identical; and
, wherein said genome of said plant cell comprises a target site comprising the first and the second non-identical recombination sites of said transfer cassette; and
,b) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites.
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Abstract
Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
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Citations
21 Claims
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1. A method for targeting the insertion of a polynucleotide of interest to a specific chromosomal site in a genome of a plant cell, said method comprising:
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a) introducing into the plant cell a transfer cassette by particle bombardment, said transfer cassette comprising said polynucleotide of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are non-identical; and
, wherein said genome of said plant cell comprises a target site comprising the first and the second non-identical recombination sites of said transfer cassette; and
,b) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification