Method for creating a reference region and a sample region on a biosensor
First Claim
1. A method for preparing a patterned surface on a biosensor, said method comprising the step of:
- utilizing at least a deposition technique to create a reference region and a sample region on the surface of said biosensor, where the deposition technique includes one of the following contact pin printing, non-contact ink jet printing, non-contact aerosol printing, capillary printing, microcontact printing, pad printing, and screen printing, silk screening, micropipetting, and spraying.
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Abstract
A method is described herein that can use any one of a number of deposition techniques to create a reference region and a sample region on a single biosensor which in the preferred embodiment is located within a single well of a microplate. The deposition techniques that can be used to help create the reference region and the sample region on a surface of the biosensor include: (1) the printing/stamping of a deactivating agent on a reactive surface of the biosensor; (2) the printing/stamping of a target molecule (target protein) on a reactive surface of the biosensor; or (3) the printing/stamping of a reactive agent on an otherwise unreactive surface of the biosensor.
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Citations
19 Claims
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1. A method for preparing a patterned surface on a biosensor, said method comprising the step of:
utilizing at least a deposition technique to create a reference region and a sample region on the surface of said biosensor, where the deposition technique includes one of the following contact pin printing, non-contact ink jet printing, non-contact aerosol printing, capillary printing, microcontact printing, pad printing, and screen printing, silk screening, micropipetting, and spraying. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for preparing a patterned surface on a biosensor, said method comprising the step of:
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coating the surface with a reactive agent; printing a deactivating agent on a predetermined area of the surface to create a reference region; and exposing the surface to target molecules wherein the target molecules bind to a defined area of the surface that does not have the printed deactivating agent to create a sample region. - View Dependent Claims (12, 13)
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14. A method for preparing a patterned surface on a biosensor, said method comprising the step of:
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coating the surface with a reactive agent; printing target molecules on a predetermined area of the surface to create a sample region; and exposing the surface to a deactivating agent to inactivate a portion of the surface that had the reactive agent exposed thereon to create a reference region. - View Dependent Claims (15, 16)
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17. A method for preparing a patterned surface on a biosensor, said method comprising the step of:
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coating the surface with a non-reactive agent; printing an activating agent on a predetermined area of the surface; exposing the surface to target molecules, where the target molecules attach to at least a portion of the surface that has the activating agent exposed thereon to create a sample region; and using the surface without the activating agent and the target molecules as a reference region. - View Dependent Claims (18, 19)
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Specification