Method and Device for Detection of Nucleic Acid Sequences
First Claim
Patent Images
1. An assay device comprising:
- a substrate and an optically transparent cover, the substrate comprisinga first surface,at least one sample receiving chamber for a liquid sample,at least one distributor channel in fluid communication with the at least one sample receiving chamber,at least one reaction chamber comprising a recess in the first surface, at least one inflow channel in fluid communication with the at least one distributor channel and the at least one reaction chamber, andat least one vent in fluid communication with the at least one reaction chamber,wherein the optically transparent cover seals the first surface, and the substrate comprises at least a portion adjacent the at least one reaction chamber and which has a thermal conductivity of about 0.25 W/m°
K. or greater.
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Abstract
A device (420) and method for real-time amplification and detection of a large number of target nucleic acids contained in a large number of biological samples is provided. The device (420) can comprise a substrate (422) and an optically transparent cover, wherein the substrate can comprise a first surface, at least one sample receiving chamber (430), a distributor channel (434), at least one reaction chamber (428), and a vent (440) for each reaction chamber. The substrate can comprise a material having a thermal conductivity of at least about 0.25 W/m° K.
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Citations
35 Claims
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1. An assay device comprising:
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a substrate and an optically transparent cover, the substrate comprising a first surface, at least one sample receiving chamber for a liquid sample, at least one distributor channel in fluid communication with the at least one sample receiving chamber, at least one reaction chamber comprising a recess in the first surface, at least one inflow channel in fluid communication with the at least one distributor channel and the at least one reaction chamber, and at least one vent in fluid communication with the at least one reaction chamber, wherein the optically transparent cover seals the first surface, and the substrate comprises at least a portion adjacent the at least one reaction chamber and which has a thermal conductivity of about 0.25 W/m°
K. or greater. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method comprising:
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introducing a liquid sample into one or more sample receiving chambers of an assay device; moving the liquid sample from the one or more sample receiving chambers through one or more channels and into a plurality of reaction chambers in the device, the plurality of reaction chambers between covered by an optically transparent cover; venting gas from the plurality of reaction chambers through a venting system in the device, the venting system being in fluid communication with the plurality of reaction chambers, while preventing liquid sample from exiting the plurality of reaction chambers; and increasing the temperature of the liquid sample in the plurality of reaction chambers at a rate of about one °
C./second or greater. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. A method comprising:
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introducing a liquid sample containing a nucleic acid sequence into one or more sample receiving chambers of an assay device; moving the liquid sample with capillary force from the one or more sample receiving chambers through one or more channels and into a plurality of reaction chambers in the device, the plurality of reaction chambers being covered by one or more optically transparent covers; venting gas from the plurality of reaction chambers through a venting system in the device, the venting system being in fluid communication with the plurality of reaction chambers, while preventing liquid sample from exiting the plurality of reaction chambers; and amplifying at least a portion of the nucleic acid sequence in one or more of the plurality of reaction chambers. - View Dependent Claims (31, 32, 33, 34, 35)
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Specification