METHOD FOR BLOCKING NON-SPECIFIC PROTEIN BINDING ON A FUNCTIONALIZED SURFACE

• US 20080213910A1
• Filed: 10/26/2007
• Published: 09/04/2008
• Est. Priority Date: 10/31/2006
• Status: Abandoned Application

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Inventors: JOGIKALMATH, Gangadhar
• Application Number:
  US11/925,593
• Publication Number:
  US 20080213910A1
• Time in Patent Office: Days
• US Class Current: 436/86
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A method of blocking non-specific protein binding on surfaces, such as protein-coated biosensor surfaces.

Claims

1. A method for reducing non-specific binding on a surface, wherein the surface is aldehyde-functionalized, amine-functionalized, or a combination thereof, comprising:

treating the surface with sugar molecules, whereby non-specific binding on the surface is reduced.

2. The method of claim 1, wherein the sugar molecules comprise disaccharides.

3. The method of claim 2, wherein the disaccharides are trehalose molecules or comprise trehalose molecules.

4. The method of claim 1, wherein the sugar molecules are dextran sulfate or comprise dextran sulfate.

5. The method of claim 1, wherein the surface is a biosensor surface.

6. The method of claim 5, wherein the biosensor is a calorimetric resonant reflectance biosensor.

7. The method of claim 1, wherein the sugar molecules comprise monosaccharides, disaccharides, polysaccharides, trisaccharides, tetrasaccharides, pentasaccharides, or a combination thereof.

8. The method of claim 1, wherein the surface is an amine-functionalized surface.

9. The method of claim 8, wherein the sugar molecules comprises lactose, glyceraldehydes, or a combination thereof.

10. The method of claim 1, wherein the sugar molecules comprise trehalose and lactose.

11. The method of claim 1, wherein the sugar molecules comprise trehalose and glyceraldehyde.

12. A biosensor comprising a plurality of specific binding substances bound to surface-attached aldehyde groups and a plurality of sugar molecules bound to surface-attached aldehyde groups.

13. The biosensor of claim 12, wherein the sugar molecules comprise disaccharides.

14. The biosensor of claim 13, wherein the disaccharides are trehalose molecules.

16. The biosensor of claim 13, wherein the disaccharides are trehalose molecules.

17. The biosensor of claim 12, wherein the sugar molecules comprise dextran.

18. The biosensor of claim 12, wherein the sugar molecules are dextran.

19. The biosensor of claim 12, wherein the specific binding substances are proteins.

20. The biosensor of claim 19, wherein the proteins comprise streptavidin.

15. The biosensor of claim 15, wherein the sugar molecules are disaccharides.

21. A package containing a biosensor with specific binding substances bound to surface-attached aldehyde groups and a storage solution comprising sugar molecules.

22. The package of claim 21, wherein the sugar molecules comprise disaccharides.

23. The package of claim 22, wherein the disaccharides comprise trehalose.

24. The package of claim 21, wherein the sugar molecules comprise dextran.

25. The package of claim 21, wherein the sugar molecules are dextran.