SAMPLE LOADING AND RECOVERY

• US 20080219890A1
• Filed: 03/05/2008
• Published: 09/11/2008
• Est. Priority Date: 08/04/2005
• Status: Abandoned Application

• Current Assignee: Fluidigm Corporation
• Original Assignee: Helicos Biosciences Corporation
• Inventors: Lawson, John, Kiani, Sepehr, Wellman, Parris
• Application Number:
  US12/043,116
• Publication Number:
  US 20080219890A1
• Time in Patent Office: Days
• US Class Current: 422/81
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 15/1484 microstructural devices

Abstract

A apparatus and method for loading a sample into a microfluidic flow cell allows for more precise loading, reduced cross-contamination, and more efficient use of samples to be analyzed. The system for loading a sample into a flow cell includes a flow cell defining a plurality of individually isolated channels through which fluid can flow. The flow cell also defines an inlet port and an outlet port for each of the channels. The system also includes a base that defines a chamber for receiving the flow cell, a cover pivotally attached to the base, and a passive vacuum source for pulling a volume through the flow cell. The method of loading a sample includes inserting the flow cell into the sample loading apparatus, placing a sample in at least one of the wells of the loading block, activating a vacuum source fluidly coupled to the outlet ports to pull the sample into the channel, and optionally aspirating the unused sample from the well.

Claims

1. A system for loading a sample into a flow cell comprising:

a housing adapted to receive a flow cell, the flow cell defining a plurality of individually isolated channels defining, an inlet port and an outlet port associated with each of the channels;

a cover adapted to secure said flow cell in said housing; and

a vacuum source for pulling a fluid volume through said flow cell.

2. The system of claim 1 further comprising a loading block defining a plurality of wells and being removably attachable to the housing and in fluid communication with the flow cell, such that each of the inlet ports corresponds to one of the wells.

3. The system of claim 1 further comprising an outlet block being removably attachable to the housing and flow cell in registration with the outlet ports.

4. The system of claim 1 wherein the outlet ports of the flow cell are fluidly coupled to the vacuum source.

5. The system of claim 1 wherein the chamber further comprises heater.

6. The system of claim 1 wherein the chamber further comprises springs to allow compression when the cover is in a closed position.

7. The system of claim 1 wherein the chamber further comprises a compressible material to allow compression when the cover is in a closed position.

8. The system of claim 1 wherein the cover includes a window section for a user to visualize the flow cell when the cover is in a closed position.

9. The system of claim 1 wherein the cover further comprises a microfluidic handling system fluidly coupled to the vacuum source.

10. The system of claim 1 wherein the cover further comprises a temperature sensor that contacts the flow cell when the cover is in a closed position.

11. The system of claim 1 further comprising a latch to secure the cover in a closed position.

12. A method of loading a sample into a flow cell comprising:

providing a flow cell defining a plurality of individually isolated channels through which fluid can flow, the flow cell defining an inlet port and an outlet port for each of the channels;

placing a loading block defining a plurality of wells on the flow cell such that each of the wells correspond to one of the inlet ports;

placing an unloading block on the flow cell in registration with the outlet ports;

inserting the flow cell into a sample loading apparatus;

placing a sample in at least one of the wells of the loading block;

activating a vacuum source fluidly coupled to the outlet ports to pull the sample into the channel.

13. The method of claim 12, further comprising monitoring the sample flowing into the channel.

14. The method of claim 13, further comprising deactivating the vacuum source after the channel is filled with the sample.

15. The method of claim 14, further comprising aspirating the remaining sample from the well.

16. The method of claim 12, further comprising deactivating the vacuum source after the channel is filled with the sample.

17. The method of claim 16, further comprising waiting a predetermined period of time for the samples to hybridize.

18. The method of claim 17, further comprising reactivating the vacuum source to evacuate the sample from the channel.

19. The method of claim 16, further comprising heating the flow cell to a predetermined temperature.

20. The method of claim 19, further comprising waiting a predetermined period of time for the samples to hybridize.

21. The method of claim 20, further comprising reactivating the vacuum source to evacuate the sample from the channel.

22. The method of claim 12, further comprising aspirating the remaining sample from the well.