Method of Treating and Preventing Infectious Diseases via Creation of a Modified Viral Particle with Immunogenic Properties
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Abstract
The present invention relates to a method for reducing the occurrence and severity of infectious diseases, especially infectious diseases in which lipid-containing infectious organisms are found in biological fluids, such as blood. The present invention employs solvents useful for extracting lipids from the lipid-containing infectious organism, thereby reducing the infectivity of the infectious organism. The present invention uses optimal solvent systems such that the lipid envelope around the viral particle is dissolved while the viral particle remains intact, resulting in a modified viral particle. The present invention also provides an autologous vaccine composition, comprising a lipid-containing infectious organism, treated with solvents to reduce the lipid content of the infectious organism, combined with a pharmaceutically acceptable carrier. The vaccine composition is administered to an animal or a human to provide protection against the lipid-containing infectious organism. The present invention further provides a simple, inexpensive and easy to use kit for delipidating fluids and for delipidation of lipid-containing organisms in a fluid.
105 Citations
27 Claims
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1-10. -10. (canceled)
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11. A method for provoking a positive immune response in an animal or human having a plurality of lipid-containing viral particles comprising the steps of:
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obtaining a fluid containing the lipid-containing viral particles from the animal or the human; contacting the fluid containing the lipid-containing viral particles with a first organic solvent capable of extracting lipid from the lipid-containing viral particles; mixing the fluid and the first organic solvent; permitting organic and aqueous phases to separate; collecting the aqueous phase containing modified viral particles with reduced lipid content; and introducing the aqueous phase containing the modified viral particles with reduced lipid content into the animal or the human wherein the modified viral particles with reduced lipid content provoke a positive immune response in the animal or the human. - View Dependent Claims (12, 13, 22, 26, 27)
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14. A method for treating a viral infection in an animal or human patient comprising:
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removing blood containing a plurality of lipid-containing infectious viral particles from the animal or the human; obtaining plasma from the blood, the plasma containing the lipid-containing infectious viral particles; contacting the plasma containing the lipid-containing infectious viral particles with a first organic solvent capable of extracting lipid from the lipid-containing infectious viral particles to produce modified viral particles having reduced lipid content; mixing the plasma and the first organic solvent; permitting organic and aqueous phases to separate; collecting the aqueous phase containing the modified viral particles; and introducing the aqueous phase containing the modified viral particles into the animal or the human wherein the modified viral particles have at least one exposed patient-specific antigen that was not exposed in the plurality of lipid-containing infectious viral particles. - View Dependent Claims (15, 16, 17)
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18-19. -19. (canceled)
- 20. A method of providing protection in an animal or a human against a viral infection comprising the step of administering to the animal or the human of an effective amount of a composition comprising modified viral particles with reduced lipid content and a pharmaceutically acceptable carrier, wherein the amount is effective to provide a protective effect against infection by the lipid-containing viral particle in the animal or the human.
- 23. The method of 11, wherein the first organic solvent is an alcohol, an ether, or a combination thereof.
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25. The method of 11, wherein the de-emulsifying agent is an ether.
Specification