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CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS IN MICROWELLS

  • US 20080220520A1
  • Filed: 02/18/2008
  • Published: 09/11/2008
  • Est. Priority Date: 11/19/2003
  • Status: Abandoned Application
First Claim
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1. A method of preparing embryonic stem cells for cryopreservation, the method comprising the steps of:

  • culturing embryonic stem cells on a first matrix in a microwell that supports growth of undifferentiated embryonic stem cells to form an embryonic stem cell colony;

    providing on the embryonic stern cell colony a second matrix that supports growth of undifferentiated embryonic stem cells to form a cryoprotective matrix-colony-matrix construct;

    exposing the construct to a cryoprotecting medium for a time sufficient to protect the viability of the cells in the matrix-colony-matrix construct; and

    replacing the cryoprotecting medium with a freezing medium.

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