Substrates and methods for selective immobilization of active molecules
First Claim
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1. A substrate having active molecules of interest disposed thereon, comprising:
- a first layer having a first surface;
a second layer disposed over a portion of the first surface;
wherein the second layer comprises a material having a substantially increased or decreased ability to bind the active molecules of interest, relative to the first layer;
active molecules of interest preferentially bound to one of the first and second layers.
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Abstract
Substrates and methods for providing increased selectivity in the immobilization of active molecules of interest in desired locations of substrates for use in analytical operations and particularly optical analytical operations.
63 Citations
26 Claims
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1. A substrate having active molecules of interest disposed thereon, comprising:
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a first layer having a first surface; a second layer disposed over a portion of the first surface; wherein the second layer comprises a material having a substantially increased or decreased ability to bind the active molecules of interest, relative to the first layer; active molecules of interest preferentially bound to one of the first and second layers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method of selectively providing active molecules of interest in a first location on a substrate, comprising:
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providing a first substrate layer having a first surface; providing a second layer disposed over a portion of the first surface, the second layer having an increased ability to bind the active molecules of interest relative to the first layer; providing a third layer disposed over at least a portion of the second layer, the third layer having a substantially descreased ability to bind the molecules of interest relative to the second layer, and a portion of the second layer remaining exposed; and contacting the substrate with the molecules of interest to coupled active molecules of interest preferentially to the portion of the second layer that is exposed, relative to an exposed portion of the first layer and the third layer.
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26. A method of analyzing an active molecule of interest, comprising:
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providing a layered substrate comprising; a first transparent layer; a second layer disposed over the first transparent layer, the second layer having a substantially increased ability to bind the active molecules of interest relative to the first transparent layer; a third layer disposed over the second layer; a plurality of apertures disposed through the second and third layers to provide an observation aperture through the transparent layer, an internal wall of the apertures exposing a portion of the second layer; and active molecules of interest preferentially bound to the portion of the second layer exposed in the apertures; observing activity of the active molecules of interest through the observation aperture in the transparent substrate.
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Specification