Dendritic cell co-stimulatory molecules
First Claim
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1. An isolated nucleic acid molecule that encodes a mammalian protein termed B7-DC that is selectively expressed on dendritic cells as compared to activated macrophages.
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Abstract
A novel costimulatory protein molecule, B7-DC, which is a member of the B7 family, is described as is DNA coding therefor and expression vectors comprising this DNA. B7-DC protein, fragments, fusion polypeptides/proteins and other functional derivatives, and transformed cells expressing B7-DC are useful in vaccine compositions and methods. Compositions and methods are disclosed for inducing potent T cell mediated responses that can be harnessed for anti-tumor and anti-viral immunity.
119 Citations
116 Claims
- 1. An isolated nucleic acid molecule that encodes a mammalian protein termed B7-DC that is selectively expressed on dendritic cells as compared to activated macrophages.
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2. (canceled)
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4-8. -8. (canceled)
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10-11. -11. (canceled)
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13. (canceled)
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16. A vector composition comprising
(a) a first recombinant expression vector having incorporated in its nucleic acid a nucleotide sequence encoding an antigen of interest against which an immune response is to be induced; - and
(b) a second recombinant expression vector having incorporated in its nucleic acid one or more nucleotide sequences encoding a co-stimulator polypeptide, at least one of which co-stimulator polypeptides is B7-DC, or is a biologically active fragment, homologue or other functional derivative thereof, wherein the expression vectors are able to co-infect or co-transfect a host cell resulting in co-expression of the antigen and the costimulator polypeptide, fragment, homologue or derivative.
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17-18. -18. (canceled)
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20-29. -29. (canceled)
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30. An isolated mammalian tumor cell transfected with an exogenous nucleic acid molecule encoding a mammalian B7-DC protein or a biologically active fragment, homologue or other functional derivative thereof, such that when said protein, fragment, homologue or derivative is expressed by said tumor cell, and said tumor cell is contacted with T cells
(i) said B7-DC protein, fragment, homologue or derivative binds to said T cells; - and
(ii) said tumor cell costimulates said T cells to proliferate and/or to produce and secrete cytokines. - View Dependent Claims (31)
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32. A polypeptide that is selectively expressed on dendritic cells as compared to activated macrophages and has the following functional properties:
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(a) binds to a binding partner on T cells; and (b) costimulates T cells to proliferate and/or to produce and secrete cytokines. - View Dependent Claims (96, 115)
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33-34. -34. (canceled)
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36-37. -37. (canceled)
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38. A B7-DC fusion polypeptide having a first fusion partner comprising all or a part of a B7-DC protein which
(i) is fused directly to a second polypeptide or, (ii) optionally, is fused to a linker peptide sequence that is fused to said second polypeptide.
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39-46. -46. (canceled)
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48. (canceled)
- 49. An antibody that is specific for an epitope of a B7-DC protein, which epitope is not present in a known member of a B7 family protein.
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50-53. -53. (canceled)
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59-60. -60. (canceled)
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62-75. -75. (canceled)
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77-82. -82. (canceled)
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84-95. -95. (canceled)
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97-110. -110. (canceled)
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103-114. -114. (canceled)
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116-120. -120. (canceled)
Specification