Test System and Method for the Detection of Analytes

• US 20080227086A1
• Filed: 01/31/2005
• Published: 09/18/2008
• Est. Priority Date: 01/30/2004
• Status: Abandoned Application

• Current Assignee: Siemens Healthcare Diagnostics Products GmbH (Siemens AG)
• Original Assignee: Siemens Healthcare Diagnostics Products GmbH (Siemens AG)
• Inventors: Vitzthum, Frank
• Application Number:
  US10/587,831
• Publication Number:
  US 20080227086A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 2525/203 incorporating a composite nucleic acid containing a polypeptide sequence other than PNA
• CPC Class Codes: C12Q 2563/125 the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode

Abstract

The invention relates to analytical test systems and analytical methods, in which molecular switches are used for the qualitative and quantitative determination of analytes in a sample, which have a broad application by means of a selection of the molecular switch suitable for the analyte. The molecular switch comprises a probe, preferably a nucleic acid or a nucleic acid derivative, coupled to a catalytic component, preferably an enzyme. The analyte induces a conformation change in the probe, which alters the accessibility for a substrate in the probe to catalytic components and the change in substrate turnover, corresponding to the change in catalytic activity, is measured.

Claims

1. An analytical test system comprising a molecular switch comprising a probe and a catalytic component.

2. The system as claimed in claim 1, wherein the probe is conjugated to the catalytic component either directly or by way of a coupling component.

3. The system as claimed in claim 2, wherein the catalytic activity of the molecular switch is changed when an analyte contacts the probe.

4. The system as claimed in claim 3, wherein the change in the catalytic activity of the molecular switch is due to a conformational change in the probe which is elicited by the analyte.

5. The system as claimed in claim 1, wherein the probe or a constituent thereof is a nucleic acid or a nucleic acid derivative.

6. The system as claimed in claim 5, wherein the nucleic acid is a ribonucleic acid, a deoxyribonucleic acid, a peptide nucleic acid, or a locked nucleic acid.

7. The system as claimed in claim 5, wherein the nucleic acid or the nucleic acid derivative is present in hybridized form.

8. The system as claimed in claim 1, wherein the probe or a constituent thereof is an oligonucleotide.

9. The system as claimed in claim 8, wherein the oligonucleotide exhibits an intramolecular hybridization.

10. The system as claimed in claim 1, wherein the catalytic component or a constituent thereof is an enzyme, an antibody, a catalytically active nucleic acids or a catalytically active nucleic acid derivative.

11. The system as claimed in claim 10, wherein the catalytically active nucleic acid or the catalytically active nucleic acid derivative is a ribonucleic acid, a deoxyribonucleic acid, a peptide nucleic acid, or a locked nucleic acid.

12. The system as claimed in claim 10, wherein the catalytic component or a constituent thereof is an enzyme.

13. A method for determining the presence or concentration of an analyte in a sample, comprising contacting a molecular switch with a sample and measuring the activity of the molecular switch to thereby determine the presence or concentration of the analyte.

16. The method of claim 13, wherein the analyte is a nucleic acid or a nucleic acid derivative.

14-15. -15. (canceled)

17. A molecular switch comprising a probe and a catalytic component.