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Circular DNA vectors for synthesis of RNA and DNA

  • US 20080227160A1
  • Filed: 09/20/2006
  • Published: 09/18/2008
  • Est. Priority Date: 04/15/1993
  • Status: Active Grant
First Claim
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1. A method for synthesizing an RNA oligonucleotide inside a cell comprising introducing into a cell a single-stranded circular oligonucleotide template comprising at least one copy of a nucleotide sequence complementary to the sequence of the RNA oligonucleotide, such that the circular oligonucleotide is processed intracellularly to yield an RNA oligonucleotide multimer comprising multiple copies of the RNA oligonucleotide.

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