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Expression monitoring by hybridization to high density oligonucleotide arrays

  • US 20080227653A1
  • Filed: 04/19/2006
  • Published: 09/18/2008
  • Est. Priority Date: 06/07/1989
  • Status: Abandoned Application
First Claim
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1. A method of monitoring RNA processing, said method comprising:

  • (a) providing a pool of target nucleic acids comprising RNA transcripts of one or more genes, or nucleic acids derived therefrom using said RNA transcripts as templates;

    (b) hybridizing said pool of target nucleic acids to an array of oligonucleotide probes immobilized on a surface, said array comprising more than 100 different oligonucleotides, at least some of which comprise control probes, wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and at least some of said oligonucleotide probes are complementary to said RNA transcripts or said nucleic acids derived therefrom using said RNA transcripts; and

    (c) quantifying the hybridization of said nucleic acids to said array, wherein said quantification is proportional to the expression level of said genes.

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