CELL ARRAY STRUCTURAL BODY AND CELL ARRAY

• US 20080227664A1
• Filed: 02/27/2008
• Published: 09/18/2008
• Est. Priority Date: 03/16/2007
• Status: Abandoned Application

• Current Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Original Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Inventors: Takahashi, Atsushi, Honma, Tsutomu, Ibii, Takahisa
• Application Number:
  US12/038,304
• Publication Number:
  US 20080227664A1
• Time in Patent Office: Days
• US Class Current: 506/39
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 41/12 of temperature controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00
• CPC Class Codes: C40B 40/02 Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
• CPC Class Codes: C40B 60/12 for screening libraries

Abstract

The present invention provides a cell array structural body containing a substrate, a plurality of micropores piercing the substrate from one surface to another surface, through which a sample cell can pass, and a capture/release unit for the sample cell on a wall surface of each micropore, as well as a cell array with such structural body detachably immobilizing sample cells in its micropores. In using the cell array structural body and the cell array for drug evaluations or the like, handling of a culture medium or a drug solution, or washing procedure is easy, and harvest of a desired cell from the cell array is easy and trustworthy.

Claims

1. A cell array structural body comprising a substrate, a plurality of micropores piercing the substrate from one surface to another surface, through which a sample cell can pass, and a capture/release unit for the sample cell on a wall surface of each micropore.

2. The cell array structural body according to claim 1, wherein the capture/release unit for the sample cell comprises as a constituent a stimuli-responsive polymer exposed on the wall surface of the micropore.

3. The cell array structural body according to claim 2, wherein the stimuli-responsive polymer is a temperature-responsive polymer.

4. The cell array structural body according to claim 3, wherein an electricity-heat converter is equipped in the vicinity of the temperature-responsive polymer.

5. The cell array structural body according to claim 4, wherein the electricity-heat converter is equipped for each micropore.

6. The cell array structural body according to claim 1, wherein the capture/release unit for the sample cell comprises as constituents electrodes to generate a non-uniform electric field.

7. The cell array structural body according to claim 6, wherein the electrodes constitute a pair comprising a plate electrode and a rod-like electrode facing each other.

8. The cell array structural body according to claim 1, wherein a cross-section of the micropore of a region between a region with the capture/release unit for the sample cell and an opening at a surface is same or larger than a cross-section of the micropore of the region with the capture/release unit for the sample cell, and a cross-section of the micropore of at least a part of a region between the region with the capture/release unit for the sample cell and an opening at another surface is smaller than the cross-section of the micropore of the region with the capture/release unit for the sample cell.

9. A cell array which comprises:

a cell array structural body comprising a substrate, a plurality of micropores piercing the substrate from one surface to another surface, through which a sample cell can pass, and a capture/release unit for the sample cell on a wall surface of each micropore; and

the sample cells retained in the micropores.